阿片类物质对小鼠抗绵羊红细胞抗体产生的影响及其机制

Effects of Opioids on Production of Anti-SRBC Antibody in Mouse and the Mechanism

本文应用体外培养免疫小鼠脾细胞,观察了吗啡、α-CAO、MENK、DADLE、强啡肽对小鼠脾细胞产生抗绵羊红细胞抗体及白三烯C_4的调节作用,结果表明:吗啡、α-CAO、MENK、DADLE与小鼠脾细胞作用48小时后,抑制脾细胞产生抗SRBC抗体,抑制百分率分别为:30～46％,28～38％,20～35％,23～55％,与对照相比均P<0.05。强啡肽与小鼠脾细胞作用48小时后,刺激脾细胞产生抗SRBC抗体,增加百分率为54～67％,与对照相比P<0.05。纳洛酮能够拮抗上述各种阿片类物质的作用。吗啡、α-CAO、MENK、DADLE抑制免疫小鼠脾细胞产生白三烯C_4,抑制百分率分别为:30％,37.5％,31.1％,35％,与对照相比均P<0.05;强啡肽刺激白三烯C_4产生,增加37％,与对照相比P<0.05.纳洛酮可以拮抗阿片类物质对白三烯C_4的产生的调节作用。

In this study, with cultured immune-mice spleen cells in vitro, it is observed that opioids can regulate production of anti-SRBC antibody and LTC4.The results indicate, Morphine, α-CAO, MENK, DADLE inhib it production of anti-SRBC antibody. The inhibition percentage is. 30-40%*, 28-38%*, 20-25%*, 23-55%'respectively. Dynorplun increases production of anti-SRBC antibody by 54-67%*. The inhibition or increase can be reversed by naloxone. Morphine,α-CAO, MENK, DADLE inhibit production of LTC4 in immune-mice spleen cells.The inhibition percentage is 30%*,37.5*, 31*, 35%* respectively. Dynorphin can increase production of LTC4 by 37%*. The opioids action can be reversed by naloxone. (*P<0.05 compaired with control).