水溶性有机介质中漆树酶催化氧化对茴香胺

Catalytic Oxidation of P-Anisidine with Rhus Laccase in Water Soluble Organic Solvents

采用分光光度法 ,考察不同水 /水溶性有机溶剂体系中漆树酶的催化氧化性能 ,以及影响反应的因素 .研究结果表明 ,漆树酶在醇溶剂中的活性要强于在非醇溶剂中 ,醇分子中羟基数量对漆树酶活性也有影响 .在温度 4 5℃ ,pH值为 7.5 ,体积比为 1∶1的水 /丙三醇溶液中 ,漆树酶的活力最高 ,活力比达 15 0 %以上 ,超出漆树酶在纯水中的活性 .以漆树酶催化氧化对茴香胺 ,采用TLC和HPLC分离纯化反应产物 根据光谱分析法 。

By adopting spectrophotometry, the authors inspected the performance of Rhus laccase catalytic oxidation in differenl water/water soluble organic solvent systems; and the factors influencing the reaction. It was found that Rhus laccase shows better activity in alcohols than in nonalcoholic organic solvent; and the number of hydroxyl groups in alcohol molecule also exerts influence on the activity of Rhus laccase. In water/glycerin solution where volume ratio of water and glycerin was 1∶1 and temperature was around 45 ℃ and pH was around 7.5, Rhus laccase showed the highest activity, which was for better than that in pure water, with an activity ratio over 150%. Then the p-anisidine was catalytically oxidized with Rhus laccase as catalyst, the reaction product was separated and purified by HPLC and TLC. According to spectrophotometry, the main product was determined to be p-azoanisol.