三氧化二砷对人肝癌细胞黏附和侵袭影响的实验研究

Study on Effect of Arsenic Trioxide on Adhesion and Invasion of Human Hepatocarcinoma Cells in Vitro

目的探讨三氧化二砷 (亚砷酸 ,As2 O3)注射液是否具有抗肝癌细胞黏附和侵袭的作用及其相关机制。方法选用人类肝癌细胞株SMMC 772 1细胞及裸鼠人类肝癌转移模型为研究对象 ,通过细胞运动迁移实验、细胞黏附实验和免疫组化方法观察As2 O3对SMMC 772 1细胞的运动迁移、与纤维粘连蛋白(FN)、内皮细胞黏附以及裸鼠人肝癌转移模型肝移植瘤表达CD4 4和MMP 2基因蛋白的影响。结果As2 O3注射液可显著抑制SMMC 772 1细胞在FN上的迁移运动和SMMC 772 1细胞与FN、内皮细胞的黏附 ,并能降低裸鼠肝移植瘤CD4 4、MMP 2的表达。结论As2 O3具有抗肝癌细胞黏附和侵袭的作用 ,其机制可能与As2 O3能降低肝癌细胞表达CD4 4和MMP 2有关。

Objective To explore that the arsenic trioxide injection (ATI) has the effect in antagonizing adhesion and invasion of human hepatocarcinoma cells (HCC), and its relevant mechanism. Methods Human hepatocellular carcinoma cell line SMMC 7721 and the high metastatic nude mice human HCC in situ transplantation model was taken as the objects of study, the effects of ATI on the SMMC 7721 cell movement and migration, its adhesion with fibronectin (FN) and endothelial cell (EC), as well as the CD44 and MMP 2 gene protein expression in transplanted tumor of the model mice were observed by means of cell movement and migration test, cell adhesion test and immunohistochemical method. Results ATI could significantly inhibit SMMC 7721 cell movement and migration on FN, adhesion with FN and EC, also could lower CD44 and MMP 2 in cancer cells. Conclusion ATI has the effects of antagonizing hepatocarcinoma cell adhesion and invasion, the mechanism may be related with the action of ATI in lowering CD44 and MMP 2 expression in cancer cells.