摘要
目的 探讨P物质 (SP)对病理性瘢痕成纤维细胞凋亡相关基因表达的影响。方法 体外培养瘢痕疙瘩、增生性瘢痕及正常皮肤成纤维细胞 ,采用细胞爬片免疫组织化学方法检测在SP及SP受体拮抗剂Spantide作用下不同成纤维细胞PCNA、bcl 2、bax的表达。结果 SP可促进不同成纤维细胞PCNA、bcl 2的表达 ,同时抑制其bax的表达。在SP作用下 ,瘢痕疙瘩成纤维细胞PCNA、bcl 2表达的增强程度及bax表达的受抑程度显著强于增生性瘢痕成纤维细胞和正常成纤维细胞 ,而增生性瘢痕成纤维细胞又强于正常成纤维细胞。Spantide可以完全或部分拮抗SP对不同成纤维细胞的作用。结论 SP通过调控成纤维细胞凋亡相关基因的表达参与病理性瘢痕形成 ,该作用由SP受体介导。
Objective To investigate the effects of substance P (SP) on the expression of apoptosis-associated genes in fibroblasts derived from pathological scars. Methods Fibroblasts derived from keloid, hypertrophic scar and normal skin were cultured separately in media containing SP and SP receptor antagonist. PCNA, bcl-2 and bax protein in fibroblasts were assessed by means of immunohistochemistry. Results SP enhanced PCNA and bcl-2 expression in all three kinds of fibroblasts, whereas, bax expression was inhibited significantly. SP inhibited the expression of bax in keloid scar fibroblasts (KSFB) more remarkably than that in hypertrophic scar fibroblasts (HSFB) or normal fibroblasts (NFB), and the effect was stronger on HSFB than on NFB. SP receptor antagonist could inhibit those effects of SP totally or partially. Conclusion SP may play an important role in the formation of pathological scars by modulating the expression of apoptosis-associated genes, which is mediated by SP receptor.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2004年第11期984-985,共2页
Medical Journal of Chinese People's Liberation Army