DNA指纹技术在可疑精子标本等鉴定中的应用

Identification of the resource suspected sperm by DNA fingerprinting

目的 :应用DNA指纹技术对精子库供精者中来源可疑的精子标本进行鉴定 ,并对供精者的后代进行亲子鉴定。方法 :分别对本研究室所涉及的 6名供精者的精子标本及其对应可疑精子标本各一份 ,同时志愿者精子和其儿子外周血各一份提取DNA ,应用PCR扩增 ,PAGE胶电泳检测DNA指纹图。结果 :通过DNA指纹图分析 ,5个可疑精子标本被确定为对应同一供精者精子标本 ,余 1例被确认为非同一供精者精子标本来源 ;志愿者和其儿子被确定为亲子关系。结论 :应用DNA指纹图技术对精子库供精者的可疑精子标本和供精者精子及对应供精者精子和后代外周血进行分析 。

Objective To identify the resource suspected sperm of donor in human sperm bank and apply the parentage testing between the donor and his offspring.Methods We took the 6 semen specimen of the donor involved and correspondently suspected semen as well as the semen of one volunteer and peripheral blood of his offspring. All specimens were amplified by PCR, and DNA fingerprint was detected by PAGE electrophoresis.Results By DNA fingerprinting we discovered that the 5 suspected sperm samples came from corresponding donors and the other sample from another. The volunteer and his offspring were identified as consanguinity.Conclusion We can identify the difference between sperm of donors and the suspected sperm of donors, and sperm of donors and the peripheral blood of their offspring exactly by DNA fingerprinting.