摘要
用农杆菌介导法获得转淀粉分枝酶基因(rbe1)水稻珍汕97B、 -32B,恢复系明恢86、明恢81和航1号.分子检测表明,rbe1已经整合到籼稻的染色体中;对转基因水稻后代遗传分离进行分析,获得初步结果.
Rice starch branching enzyme gene rbe1 under the control of promotor Gt1 in sense orientation was introduced into several varieties of indica rice (Zhenshan 97B; Ⅱ-32B; restorer line Minghui 86, Minghui 81 and Hang No.1) by Agrobacterium-mediated transformation after comparing several influence factors, including callus state, antibiotic type and concentration, and hygromycin content. PCR and Southern blotting analysis showed that rbe1 gene had been integrated into indica rice genome.
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2004年第1期64-67,共4页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
国家转基因植物研究与产业化专项(J2000-B-004)
福建省重大科技资助项目(2000-Z-023
2001-Z-065
2003N002).
