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缺氧诱导因子1-α在酒精性肝病形成中的表达 被引量:9

Hypoxia inducible factor 1-α mRNA expression in alcoholic liver disease
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摘要 目的 研究缺氧诱导因子1-α(HIF1-α)在洒精性肝病动物模型的表达情况,探讨其与酒精性肝病的关系。方法 采用酒精灌胃法建立酒精性肝病动物模型,应用逆转录聚合酶链反应(RT-PCR)法和免疫组织化学染色法检测大鼠肝组织HIF1-α mRNA和蛋白水平。结果 RT-PCR结果显示模型组大鼠HIF1-α mRNA表达阳性率为62.5%(5/8),对照组为16.7%(2/12),x^2=3.94,P<0.05。两组大鼠肝脏HIF1-α多克隆抗体免疫组织化学染色评分分别为3.13±0.83和0.83±1.27,差异有非常显著必,t=4.88,P<0.01。结论 HIF1-α在酒精性肝病动物模型的表达明显增高,缺氧是酒精性肝病的发病机制之一。 Objective To investigate the effect of hypoxia on chronic alcoholic liver disease. Methods Tweenty four male Sprague-Dawley rats were randomly into two groups. The alcohol group(n=12) was fed 56% (V/V) of ethanol once per day by gastric infusion at 8 g/kg body weight for 24 weeks. The control group(n=12) was gastrically infused with normal saline with the same dose. At the end of 24 weeks, a blood sample was collected for determination of hepatic enzymes and then the rat was killed. Liver specimens were obtained for immunohistochemical staining and frozen at-80℃ used for RT-PCR. Results Serum alanine aminotransferase (ALT) and aspartate aminotransferase(AST) activity increased significantly compared to the control group(P<0.01). A significant elevation in the expression of HIF1-α in liver of alcohol group was found compared to the control group(P<0.05). Conclusion Hypoxia inducible factor 1-α expression was activated by ethanol-induced injury. This information suggested that hypoxia was involved in mechanism of alcoholic liver disease.
出处 《中华肝脏病杂志》 CAS CSCD 2004年第7期417-419,共3页 Chinese Journal of Hepatology
关键词 缺氧诱导因子1-Α 酒精性肝病 动物模型 检测 发病机制 Alcoholic liver diseases Hypoxia inducible factor Ⅰ- α
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