不同温郁金提取物抑制胃癌细胞SGC-7901增殖和诱导凋亡的实验研究

The extract of common turmeric induces apoptosis of gastric carcinoma cell line SGC-7901

目的研究不同温郁金提取物对人胃癌细胞SGC-7901生长的抑制作用,及其抑瘤作用与凋亡之间的关系。方法制备温郁金水提物、石油醚提取物(醚提物)和乙醇提取物(醇提物),采用MTT法,测定3种提取物作用下的细胞增殖率。应用HE染色、电镜及流式细胞仪分析技术,观察温郁金作用下肿瘤细胞凋亡的形态学改变及凋亡率。结果温郁金水提物、醚提物和醇提物对胃癌细胞的生长有抑制作用,体外最大抑制率分别达到34.4%、49.4%和73.1%。细胞形态学检查见典型的凋亡改变。流式细胞仪测得典型的凋亡峰。结论温郁金水提物、醚提物和醇提物对人胃癌SGC-7901细胞生长有抑制作用,且醚提物和醇提物的抑瘤作用可能与诱导细胞凋亡有关。

Objective To examine whether the extract of common turmeric (Wenyujin) induce apoptosis and inhibit growth of gastric carcinoma cell line SGC-7901 in vitro. Methods Common turmeric was extracted by water, petroleum ether and ethanol. The extract of common turmeric was added to the medium of gastric carcinoma cell line SGC-7901 cultured in vitro. The proliferation of cells was detected by MTT test and direct counting method. Cell apoptotic index was examined by flow cytometry analysis and cell morphology was observed under oil and electron microscopy respectively. Results When compared with controls, tumor cell growth was significantly inhibited by extract of common turmeric decocted by water, petroleum ether and ethanol. The MTT test showed the inhibition rate was 34.4%, 49.4%和73.1% respectively. Apoptotic peak was found in cells treated with extract of common turmeric decocted by petroleum ether and ethanol. Conclusion Extract of common turmeric decocted by water, petroleum ether and ethanol inhibited growth of human gastric carcinoma cell line SGC-7901 in vitro. Apoptosis is one of the mechanisms of proliferation inhibition of tumor cells.