摘要
目的 探讨大鼠脑缺血再灌流后Bcl- 2蛋白、Caspase - 3mRNA的表达及炎性细胞浸润与神经细胞凋亡的关系。方法 将 5 4只Wistar大鼠随机分为二组 :假手术组 ,缺血再灌流组。采用原位末端标记 (TUNEL)、免疫组化和原位杂交技术分别观察脑缺血再灌流后不同时间点神经细胞凋亡及损伤的变化与Bcl- 2、Caspase - 3mRNA表达。 结果 Bcl- 2表达于缺血再灌流 12～ 2 4h达高峰 ,再灌流 2～ 4d呈下降趋势 ,至 16d略高于假手术组 ;Caspase- 3mRNA于缺血再灌流 12～ 2 4h达高峰 ,2～ 4d呈降低趋势 ,至 16d略高于假手术组。结论 脑缺血再灌流后细胞凋亡介导神经细胞损伤、坏死是一个渐进的动态演变过程。Bcl- 2蛋白、Caspase-
Objective To explore relationship of the expression of Bcl-2 protein and Caspase-3 mRNA and the infiltration of inflammation cells and the neuron apoptosis after cerebral ischemia reperfussion in rats. Methods 54 rats were divided randomly into two groups: sham-operative group and ischemia reperfusion group. The variations of neuron apoptosis and neuron injury on the different time points after cerebral ischemia reperfussion in rats were observed with the method of deoxynucleotidy 1 tranferase mediated dUTP-flourescein nick end-labelig (TUNEL) assay. The expression of Bcl-2 protein with the method of immunchiochemistry and the expression of Caspase-3 mRNA with the method of in situ hybridization. Results It showed that expression of Bcl-2 protein reached a peak at 12～ 24h after reperfussion and begin to decrease at 2～4d .but the numbers of the positive cells were still in high level after 16d compared with the sham-operated group, The expression of Caspase-3 mRNA reached a peak at 12～24h after reperfusion and decreased at 2～4d, but the number of the positive cells was still in high level after 16d compared with the sham-operated group. Conclusions The neuron apoptosis guide in injury and necrosis was a dynamic ongoing development process after cerebral ischemia reperfussion in rats. The expression of Bcl-2 protein should inhibit apoptosis, Caspase-3 mRNA should guide in neuron injury play an very important role.
出处
《神经疾病与精神卫生》
2004年第4期252-255,共4页
Journal of Neuroscience and Mental Health
