鳗鱼中恩诺沙星残留量的酶联免疫检测方法

Determination of Remained Enrofloxacin in Eel by ELISA

运用酶联免疫分析方法测定鳗鱼中恩诺沙星残留。测试曲线线性范围为0.3～10μg/kg,最低检测限为3μg/kg。向样品中分别添加25、50、100μg/kg 3个浓度水平的恩诺沙星, 平均回收率分别为74.5%、76.6%和66.5%,批内变异系数为7.62%～14.31%,批间变异系数为6.03%～7.00%,灵敏度达到0.3μg/kg。特异性试验结果表明与喹诺酮类中的其它药物以及其它类抗生素均无交叉反应。

Remained Enrofloxacin in eel were determined by ELISA. The calibration curve should be virtually linear in the rangeof 0.3～10μg/kg. The limit of detection (LOD) is 3μg/kg. Adding Enrofloxacin to eel samples, the concentration is 25, 50 and100μg/kg separately. Average recovery rate is 74.5%, 76.6%, and 66.5% separately. Coefficient of variation in same batch is7.62%～14.31%. Coefficient of variation in different batch is 6.03%～7.00%. Sensitivity is 0.3μg/kg. The rusult of special testis as following: No cross-reactivities happened to other drugs.