分光光度法测定阿奇霉素注射液的含量

Determination the content of Azithromycin Injection by spectrophotometry

目的 建立分光光度法测定阿奇霉素注射液的含量。方法 精取阿奇霉素注射液适量 ,加 0 .1mol·L-1盐酸溶解并稀释至一定浓度 ,加入 85 %硫酸 ,涡旋混合 ,静置显色 ;在 4 82nm波长处按拟定方法测定。结果 浓度在 2 0～ 70mg·L-1范围内呈线性关系 ,回归方程为 :A =0 .0 0 932 C+0 .0 2 18,r=0 .9999;平均回收率 10 1.33% (n=9) ,日内、日间精密度 RSD<1%。结论 本法准确、简便、快速 ,可用于阿奇霉素注射液的质量控制。

Aim To establish a method for determination the content of Azithromycin Injection.Methods The content in the injection was determined at 482nm by spectrophotometry after being diluted in 0.1mol·L -1 hydrochloric acid,then reacted with sulfuric acid to be colored.Results The linear relation was in the range of 20～70mg·L -1 of Aizthromycin r=0.999 9.The average recovery rate was 101.33%(n=9).The intra-day precision and the inter-day precision were less than 1%.Conclusion The method was accurate,simple,quick and suitable for the quality control of Azithromycin Injection.