流动注射抑制化学发光法测定吲达帕胺

Flow injection analysis of indapamide by inhibition chemiluminescence

在碱性介质中,吲达帕胺对N 溴代琥珀酰亚胺(NBS) 荧光素发光体系具有很强的抑制作用,并且其抑制化学发光强度与吲达帕胺的质量浓度在一定范围内呈良好的线性关系,基于此,建立了测定痕量吲达帕胺的流动注射化学发光分析法。线性范围为0.01～5.0μg mL,检出限为(3σ)4.7ng mL,对0.1μg mL的吲达帕胺连续测定11次,相对标准偏差为2.6%。将本方法用于片剂中吲达帕胺的分析,并与药典标准方法测定结果一致。

A new method for the determination of indapamide with flow injection chemiluminescence was described. It was based on the inhibition of chemiluminescence reaction of NBS-fluorescein by indapamide in sodium hydroxide medium. The linear range for determination of indapamide was 0.01～5.0 μg/mL with the relative standard deviation of 2.6% (c=0.1 μg/mL, n=11). The detection limit was 4.7 ng/mL(3σ). The method was used to determine indapamine in pharmaceutical preparation samples with satisfactory results.