甘草对醋酸铅诱发小鼠精子畸形的抑制作用

Inhibitory effects of Glycyrrhiza uralensis on sperm abnormalities induced by lead acetate in mice

目的 :观察中草药甘草对醋酸铅 [Pb (CH3COO) 2 ]诱发小鼠精子畸形的抑制作用。方法 :6 5只小鼠随机分成阴性对照组 (N· S)、阳性对照组 (MMC1.0 mg· kg- 1 )、 Pb (CH3COO) 2 诱变实验组 (4 0、 2 0和10 m g· kg- 1 ,相当于 1/ 3、1/ 6和 1/ 12 L D50 )、甘草诱变实验组 (1.2 5、 2 .5 0、 5 .0 0和 10 .0 0 g· kg- 1 ,相当于人 5×、10×、2 0×和 4 0×临床常用剂量 )、甘草抗诱变组 [(Pb (CH3COO) 2 2 0 mg· kg- 1 +甘草各剂量组 ) ],每组 5只动物。MMC和 Pb (CH3COO) 2 腹腔注射给药 ,其他药物均为灌胃给药 ,抗诱变实验组甘草和 Pb(CH3COO) 2 同时给药 ,每天 1次 ,连续 5 d。首次给药后 5周末 ,以颈椎脱臼法处死小鼠 ,取附睾 ,按 Wyrobek方法制片。结果 :甘草各剂量组 (1.2 5、 2 .5 0、 5 .0 0和 10 .0 0 g· kg- 1 )所诱发的小鼠精子畸形频率 (% ) (1.2 0± 0 .2 2、 1.38± 0 .2 7、1.5 2± 0 .2 5及 1.32± 0 .30 )与阴性对照组小鼠精子畸形频率 (1.2 8± 0 .18) %比较 ,差异无显著性 (P>0 .0 5 )。但甘草各剂量 (1.2 5、 2 .5 0、 5 .0 0及 10 .0 0 g· kg- 1 )与 Pb (CH3COO) 2 (2 0 mg· kg- 1 )一同给药时 ,可使Pb (CH3COO) 2 所诱发的小鼠精子畸形频率明显降低 ,即 (3.2 8± 0 .2

Objective To examine the inhibitory effects of Glycyrrhiza uralensis on sperm abnormalities induced by lead acetate,Pb(CH 3COO) 2, in mice.Methods Sixty-five mice were randomly divided into groups: negative control (N·S), positive control(MMC 1.0 mg·kg -1 ), lead acetate mutagenesis groups(40,20, and 10 mg·kg -1 ,which were matched 1/3,1/6, and 1/12 LD 50 ),Glycyrrhizia uralensis mutagenesis groups (1.25,2.50,5.00, and 10.00 g·kg -1 ,which were matched 5×,10×,20×, and 40× clinical commonly used dosage of a person),Glycyrrhizia uralensis antimutagenesis groups (lead acetate 20 mg·kg -1 + each Glycyrrhizia uralensis dosage groups),5 mice per group. MMC and lead acetate were given by abdominal cavity injection, other drugs were given by perfusing stomach. Glycyrrhizia uralensis and lead acetate were given simultaneously in antimutagenesis groups,once a day for 5 days successively. At the 5th weekend after the first administration,the mice were killed by cervical vertebra dislocation whose epididymidis were drawed and sheets were produced in Wyrobek method.Results Sperm abnormality frequency (%) was no significant difference between tested groups(1.20±0.22,1.38±0.27,1.52±0.25,and 1.32±0.30)and control group (1.28±0.18) (P>0.05), but the frequency of abnormal sperms induced by lead acetate (%)(5.12±0.38) was reduced significantly by intragastrial perfusion of Glycyrrhiza uralensis and lead acetate (3.28±0.24, 3.06± 0.29,2.32±0.31, and 1.84±0.27)at the same time (P<0.01). Conclusion Glycyrrhiza uralensis can′t induce the sperm abnormality of mice,but shows a protective effect, namely antimutagenesis, on genetic materials in mice.