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PPARγ配体调控人胆囊上皮细胞炎性反应的实验初探

A Preliminary Experimental Study of PPAR γ Ligands Regulate the Inflammatory Response of Human Gallbladder Epithelial Cells
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摘要 目的 :初步探讨PPARγ配体能否抑制人胆囊上皮细胞的炎症反应。方法 :行胆囊上皮细胞原代培养并鉴定 ,根据细胞生长曲线 ,在其生长高峰期 (第 5天 ) ,用 0、 10 μmol/L、 2 0 μmol/L、 30 μmol/L、 5 0 μmol/L、10 0 μmol/L的噻格列酮 (PPARγ配体之一 )处理细胞 2 4小时 ,再用人白细胞介素 (hIL) - 1β5ng/ml刺激 ,2小时后用放射免疫法测量细胞上清液中IL - 8的浓度。对 6个组别的IL - 8浓度采用双因素方差分析 ,两两比较采用最小显著差法 (LSD) ,检验水准α=0 0 5。结果与对照组相比 ,各实验组IL - 8的表达均被抑制 ,差异有统计学意义 (P <0 0 1) ,实验组 5 (10 0 μmol/L)的抑制作用最明显。结论 :PPARγ配体可抑制炎性因子IL - 8在胆囊上皮细胞中的表达 。 Objective:To study the regulatory ability of PPARγ ligands to the inflammatory response of human gallbladder epithelial cells.Methods:Culture human gallbladder epithelial cells and identify them.Cells are treated for 24 hours with 0, 10μmol/L,20μmol/L,30μmol/L,50μmol/L,100μmol/L of Ciglitazone during cellular growth peak(5 th. day),then stimulated them with hIL-1β5ng/ml for 2 hours and measure the concentration of IL-8 in the cellular supernatants by Riadioimmunoassay.All datas are analysed by the statistical mean of two-way ANOVA and LSD.α=0.05.Results:Contrasted with control group, the expression of IL-8 in each test group are inhibited(P<0.01).The effect of test group 5(100μmol/L)is the most prominent.Conclusions:PPARγligands can inhibit the expression of IL-8 in human gallbladder epithelial cells and probably produce effect in the regulation of cholecystic inflammation.
出处 《华西医学》 CAS 2004年第4期632-633,共2页 West China Medical Journal
基金 教育部留学回国人员科研启动基金资助项目 (项目编号 :[2 0 0 1 ] 345)
关键词 PPARΓ配体 上皮细胞 IL-8 胆囊 炎性反应 实验 表达 调控 原代培养 细胞生长 PPARγ ligand gallbladder epithelial cells IL-8 cholecystitis (Received date:2004-04-12)
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