黄芪多糖对NOD鼠胰岛细胞因子基因表达的影响

Effects of Astragalus Polysaccharides of Gene Expression of Cytokines of NOD Mice's lslets

目的 探讨黄芪多糖 (APS)以NOD小鼠 1型糖尿病免疫干预的分子机制。方法 应用RT CPR技术分别检测APS处理组和对照组NOD小鼠所有胰腺组织内 15条细胞因子mRNA的表达水平。结果 与对照组相比 ,APS组IL 1β、IL 2、IL 6、IL 12、TNF α、INF γ、Fas、iNOS的mRNA表达水平明显下调 ,IL 4、IL 5、IL 10、TGF β、Bcl 2、SOD的mRNA表达水平明显上调。结论 APS能纠正NOD小鼠Th1/Th2型细胞 /细胞因子的免疫失衡状态 ,预防或延缓

Purpose: To investigate the molecular mechanism of the preventive effects of astragalus polysacharide (APS) on the onset of type1 diabetes mellitus (DM) in non-obese diabetic (NOD) mice. Methods: We used RT-PCR to study the mRNA expression of 15 cytokines in NOD mice's islet cells of APS group and control group. Results: As compared to control group, the mRNA expression of IL-1β, IL-2, IL-6, IL-12, TNF-α, INF-γ, Fas, iNOS was lower in NOD mice's islet cells of APS group, while the mRNA expression of IL-4input commas IL-5input commas IL-10input commas TGF-βinput commas Bcl-2input commas SOD was higher in APS group. Conclusions: It proves the preventive effects of Astragalus Polysaccharide on the onset of type 1 diabetes in NOD mice by correcting the imbalance of Th1/Th2 cytokines.