纳米As_2O_3磁性脂质体的制备及表征

Preparation and evaluation of nano-magnetoliposomes containing As2O3

目的:制备将药物治疗与热疗相结合的靶向抗癌药物新剂型-As2O3磁性脂质体.方法:用改良的湿化学法制备MnxZn(1-x)Fe2O4(锰锌铁氧体)纳米磁性粒子,运用透射电镜和PE热分析系统进行表征,在高频交变磁场下进行体外加热试验,MTT实验检测其细胞毒性;采用薄膜-超声法加高速搅拌制备纳米As2O3磁性脂质体,用透射电镜、图像分析系统和能谱仪对其进行表征,原子荧光光度计检验脂质体中As2O3的包封率.结果:MnxZn(1-x)Fe2O4纳米磁性粒子近似球形,粒径20～40 nm,无细胞毒性,居里温度随锰锌比例的不同分布在97℃～140℃之间,其磁流体在高频交变磁场下可升温至35℃～47℃并保持恒定.以此磁性材料为载体制成的As2O3磁性脂质体的平均粒径为(182±125)nm,其中含有As2O3和MnxZn(1-x)Fe2O4的成份,药物包封率达到71.16%.结论:MnxZn(1-x)Fe2O4纳米粒子是制备医用磁性脂质体的良好载体,采用薄膜-超声法加高速搅拌可制备纳米级As2O3磁性脂质体.

Objective:To preparate nano-magnetoliposomes containing As2O3, a completely new form of drug for the targeted chemotherapy and thermotherapy to cancer cells. Metheds: The Manganese-zinc ferrite nanoparticles(Mn1-xZnxFe2O4) were prepared by improved wet-chemical reaction. Their characteristics were measured by transmission electron microscope(TEM) and PE thermal analysis system. The radiofrequence-induced hyperthermia was explored in vitro, and their cytotoxicity was measured by the means of MTT. The nano-magnetoliposomes were obtained by the combined methods of rotatory film, ultrasonic and churning at high speed. Their characteristics were measured by TEM, computer magic image analysis system and energy dispersive spectrometer(EDS). The ratio of envelopment of As2O3 was measured by atom fluorescence spectrometer. Results: The average diameter of magnetic particles was about 20-40 nm, round, the cytotoxicity of Mn1-xZnxFe2O4 was 0 class. The Curie temperature(Tc) of magnetic material varied from 97℃ to 140℃ according to different percentages of zinc and manganese in Mn1-xZnxFe2O4. The temperature of magnetic fluid could rise to 35℃-47℃ and keep stable in the high frequency alternating electromagnetic field. The nano-magneticliposomes contained As2O3 and Mn-Zn ferrite. The average diameter of the liposomes was(182 ± 125)nm. The envelopment ratio of As2O3 was 71.16%. Conclusion: The nano-particles of Mn1-xZnxFe2O4 are perfect carriers for preparating magneticliposomes; Nano-magnetic-liposomes containing As2O3 have been successfully preparated.