黑藻(Hydrillaverticillata)对铜绿微囊藻(Microcystisaeruginosa)抑制作用

The Inhibition of Hydrilla verticillata on Microcystis aeruginosa

采用黑藻(Hydrilla verticillata)和铜绿微囊藻(Microcystis aeruginosa)共同培养的实验方法,追踪测定微囊藻的生长量、光合速率等相关生理生化指标以及观察藻细胞超微结构变化,研究黑藻对铜绿微囊藻的抑制作用及其机制.结果表明,黑藻对铜绿微囊藻的生长有明显的抑制作用,表现为藻细胞生长量显著降低,细胞超微结构进行性损伤直至细胞解体,藻体叶绿素a含量和光合速率急剧下降,呼吸速率、超氧物歧化酶(SOD)活性均呈现先升高后下降的趋势,膜脂过氧化产物丙二醛(MDA)的积累量与膜的损伤程度相一致.在黑藻养殖水中初步分离得到黑藻分泌的抑藻物质.

In this paper, Microcystis aeruginosa co-cultivated with Hydrilla verticillata was used to study the inhibitory effect of Hydrilla verticillata on Microcystic aeruginosa and its mechanism. The results indicated that Microcystis aeruginosa growth was obviously inhibited by Hydrilla verticil-lat. Its ultra-structure was destroyed and finally disintegrated; chlorophyll-a content, photosyn-thetic rate declined evidently from the beginning; whereas respiratory rate and SOD activity increased firstly, but decreased as treatment time went on; MDA accumulation was consistent with the degrees of its cell destruction. Hydrilla verticillata culture had the same inhibitory effect on Microcystic aeruginosa. The crude excretion from Hydrilla verticillata culture which had the inhibitory effect on Microcystis aeruginosa was obtained.