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土壤无机阴离子的毛细管电泳分析 被引量:18

QUANTIFICATION OF INORGANIC ANIONS IN SOIL BY CAPILLARY ELECTROPHORESIS
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摘要 采用以铬酸钠为背景电解质、十六烷基三甲基溴化铵为电渗流改性剂的间接紫外检测法电泳系统 ,研究了分离电压、缓冲液的酸碱性等毛细管电泳测定氯离子、硫酸根、硝酸根和磷酸根的基本条件 ,可在 5min内对以上 4个离子实现准确定量。测定了 5个采自全国不同地区的土壤样品水提取液中的无机阴离子。结果显示 ,毛细管电泳可对提取液中的氯离子、硫酸根和硝酸根实现较准确的定量 ,测定结果与离子色谱比较一致 ;对硝态氮的测定结果与KCl提取 流动分析结果相关性达到p <0 10水平 ,表明测定结果可在一定程度上反映土壤氮素养分状况。但土壤用水静止提取 2 4h ,提取液中的磷未达到毛细管电泳和离子色谱法的检测限 。 Compared with ion chromatography (IC), for inorganic ion analysis capillary electrophoresis (CE) has its advantages, such as low consumption of reagent and time and low cost and its disadvantages, like low reproducibility and limited sensitivity. Analysis of soil aqueous extract for inorganic ions by CE is one of the fields that have been explored, however, so far the result of CE has not been matched to those of the popularly used methods, such as flow analysis (FA) of nitrate. This work focused on further study of parameters affecting the CE analysis of inorganic anions in soil, and comparison of its result with those of IC and FA. With the aid of the electrophoresis system using sodium chromate as the background electrolyte, cetyltrimethyl ammonium bromide (CTAB) as the electroosmotic flow modifier and indirect ultraviolet detection method, length of soil extraction duration,effect of separation voltage and pH of the working buffer of capillary electrophoresis (CE) on quantification of inorganic anions were investigated. Using the chosen analytical condition, chloride, sulfate, nitrate and phosphate can be quantified in less than 5 min. Based on these results, chloride, sulfate and nitrate in soil water extract were analyzed by CE in five soil samples from different places in China, the results were consistent with that of IC. However, phosphate in the soil extract after extracting for 24 h without agitation was still under the limit of detection (LOD) of CE and IC, and the CE is relatively lower in precision compared with IC. A positive linear correlation of p<0.10 was found between soil nitrate extracted with water, determined by CE and that extracted with 1 mol L -1 KCl, determined with FA.
出处 《土壤学报》 CAS CSCD 北大核心 2004年第6期881-888,共8页 Acta Pedologica Sinica
基金 中国科学院创新项目 (KZCX2 40 4) 国家高新技术发展计划 ( 863课题 ) ( 2 0 0 3AA2 0 90 10 ) 国家重点基础研究发展规划 ( 973项目 )(G19990 1180 3 )资助
关键词 土壤氮素 养分状况 硝态氮 土壤样品 测定结果 硝酸根 提取液 毛细管电泳 电渗流 离子色谱 Soil Capillary electrophoresis Inorganic anion Quantification
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