摘要
目的:研究甘草甜素脂质体对急性酒精(乙醇)性肝损伤的保护作用,为肝脏保护的康复措施介入提供实验依据。方法:采用ICR雌性小鼠,随机分为对照组,模型组和甘草甜素3个剂量组。通过预先给予各组小鼠甘草甜素脂质体,剂量分别为0.25,0.5,1.0mL/kg,30d后各剂量组及模型组小鼠经口一次给予乙醇6.5g/kg,建立急性肝损伤模型,观察甘草甜素脂质体对急性酒精性肝损伤小鼠肝脏脂质过氧化反应和肝细胞脂肪变性的影响。结果:给予乙醇溶液16h后模型组的肝过氧化反应增强,肝组织匀浆中丙二醛含量犤(5.2±1.6)μmol/g犦明显高于对照组犤(2.2±0.7)μmol/g犦(P<0.01)。甘草甜素各剂量组小鼠肝组织丙二醛含量明显低于模型组,差异有显著性意义(P<0.01);甘草甜素组的肝组织还原性谷胱甘肽含量均高于模型组,差异有非常显著性意义(P<0.01)。给予乙醇后肝组织脂肪酸代谢障碍,三酰甘油合成增加,甘草甜素对三酰甘油的升高有明显的抑制作用(P<0.01),且具有剂量反应关系。肝脏病理学组织切片分析表明甘草甜素各剂量组肝细胞脂肪变性程度减轻。结论:甘草甜素脂质体能对抗酒精中毒引起的肝脂质过氧化损伤,具有有效的保护作用。
AIM:To investigated the effect of liposomal glycyrrhizin on ethanol induced acute liver injury so as to provide an experimental evidence for hepatoprotective intervention.<METHODS:Male ICR mice were randomly divided into control group,model group and three glycyrrhizin treated groups by feeding glycyrrhizin orally at the doses of 0.25,0.5 and 1.0 mL/kg,respectively before ethanol induction.Thirty days later,the mice in model group and three glycyrrhizin treated groups of mice were administered with one off 6.5 g/kg ethanol orally to establish mouse model of acute hepatic injury.The effects of liposomal glycyrrhizin on the hepatic lipid peroxidation reaction and the fatty degeneration of hepatocytes in rats with ethanol induced acute injury were observed.<RESULTS:The hepatic lipid peroxidation reaction was promoted 16 hours after administration of ethanol in model group.The level of malondialdehyde(MDA) in hepatic homogenate of mice in model group was (5.2 ± 1.6) μ mol/g,significantly higher than that of mice in control group[(2.2± 0.7) μ mol/g](P< 0.01),and it was significantly lower in the three glycyrrhizin treated groups than in model group(P< 0.01),but the level of hepatic educed glutathione(GSH) in the three glycyrrhizin treated groups was all significantly higher that in model group(P< 0.01).Due to induction of ethanol,hepatic fatty acid metabolism was disordered,synthesis of triglyceride(TG) was promoted,and liposomal glycyrrhizin inhibited the increasing level of TG(P< 0.01) in a dose response relationship.The analysis of pathological tissue sections showed that the fatty degeneration of hepatocytes in glycyrrhizin treated groups was relieved.<CONCLUSION:Liposomal glycyrrhizin attenuated ethanol induced hepatic lipid peroxidation injury and had potential protective effects on ethanol induced acute liver injury.
出处
《中国临床康复》
CSCD
2004年第36期8260-8261,共2页
Chinese Journal of Clinical Rehabilitation