杜仲叶绿原酸提取纯化工艺的研究

Study on Technique of Extraction and Purifying Chlorogenic Acid in Eucommia ulmoides Oliver Leaves

以乙醇为溶剂提取杜仲叶绿原酸 ,采用响应面分析法优化工艺条件 ,其最佳工艺条件为 :乙醇浓度5 1 7% ,提取温度 5 5 8℃ ,料液比 1∶12 8,提取时间 2h。通过对 6种树脂进行静态吸附实验 ,选出NKA Ⅱ树脂为吸附分离杜仲叶绿原酸的最佳树脂 ,最佳吸附 解吸条件 :吸附流速 2ml/min ,原液过柱吸附 2次 ,最佳吸附剂为4 0 %乙醇。

Ethanol was used as solvent to extract chlorogenic acid from leaves of Eucommia ulmoides Oliver leaves. The optimum conditions are obtained by response surface analytical method Optimized conditions for the extraction of chlorogenic acid were obtained: ethanol 51 7%,extraction temperature 55 8℃,ratio of material to liquor 1∶12 8,extraction time 2h The static adsorption tests are conducted among six kinds of macroporous resins to select NKA-Ⅱ resin as a optimum resin to adsorb the chlorogenic acid in Eucommia ulmoides Oliver leaves The optimum adsortion-elution conditions are as follow∶adsorption flow rate is 2ml/min,crude liquid flew twice through the adsorption column,the optimum adsorbent is 40% ethanol