食管癌及癌前病变组织中hTERT基因的表达

Expressions of human telomerase reverse transcriptase gene in cancer and pre-cancer tissues of esophagus

目的:探讨肿瘤相关基因端粒酶逆转录酶(hTERT)基因在食管癌及癌前病变组织中的表达及与细胞增生核抗原Ki-67的关系;探讨hTERT基因对食管癌早期诊断及食管癌预后判定的价值.方法:采用原位杂交法检测食管癌组织42例、不典型增生组织37例及正常食管组织12例中hTERTmRNA的表达;用免疫组化S-P法检测Ki-67抗原表达.结果:在正常食管黏膜、不典型增生、食管癌组织中,hTERTmRNA的阳性表达率分别为0%,48.6%,83.3%;Ki-67抗原的阳性表达率分别为0%,56.8%,88.1%;癌组织与不典型增生组织中hTERTmRNA与Ki-67抗原阳性表达率较正常食管黏膜差异有显著性(P<0.01);癌组织中hTERTmRNA与Ki-67抗原阳性表达率较不典型增生组织差异有显著性(P<0.01);食管癌组织中hTERTmRNA与Ki-67抗原表达与临床分期、淋巴结转移有关(P<0.05);结果还显示hTERTmRNA和Ki-67抗原表达具有显著相关性(P<0.05).结论:端粒酶逆转录酶基因的异常表达在食管癌的发生、发展过程中具有重要作用,hTERTmRNA的表达与细胞增生活性有关;hTERT基因表达的检测有助于食管癌的早期诊断以及食管癌预后的判定.

AIM: To investigate the expressions of human telomerase reverse transcriptase (hTERT) gene in cancer and pre cancer tissues of esophagus and its relation with cellular proliferation antigen Ki-67 and to investigate the value of hTERT in early diagnosis for esophageal cancer as well as predication of prognosis. METHODS: In situ hybridization was adopted to detect the expressions of hTERTmRNA in tissues of esophageal cancer (42 cases), atypical hyperplasia (37 cases), and normal esophagus(12 cases). Ki-67 antigen was detected by im-munohistochemical methods. RESULTS: The positive rates of hTERTmRNA in tissues of normal mucosa, atypical hyperplasia and cancers were 0.0%, 48.6%, and 83.3% while rates of Ki-67 antigen were 0%, 56.8%, and 88.1% respectively. The expressions of hTERTmRNA and Ki-67 were significantly increased in tissues of hyperplasia and cancer, compared with those in the normal tissues (P<0.01); Expressions of hTERTmRNA and Ki-67 antigen between hyperplasia and cancer also had significant differences (P <0.01). Expressions of hTERTmRNA and Ki-67 antigen significantly related to clinical staging of esophageal cancer and metastasis of lymph node (P<0.05). Meanwhile, there was significant relation between expression of hTERTmRNA and Ki-67 antigen (P <0.05). CONCLUSION: Over-expression of hTERT gene may play an important role during carcinogenesis and progression of esophageal cancer; Expression of hTERTmRNA is rela tive to cellular proliferating activity. The detection of hTERT will be helpful for early diagnosis and prognosis evaluation of esophageal cancer.