摘要
目的:观察己酮可可碱(pentoxifylline,PTX)对血吸虫病肝纤维化小鼠肝组织中凋亡相关基因Bc1-2,Bax表达的影响.方法:建立小鼠血吸虫肝纤维化模型,将小鼠随机分为4组:(1)感染对照组,(2)高剂量PTX治疗组(360g/kg/d),(3)低剂量PTX治疗组(180g/kg/d),(4)吡喹酮治疗组(500g/kg/d).观察4组小鼠治疗8wk后肝组织中Bc1一2,Bax的表达水平,同时观察肝组织的病理学变化.结果:高剂量PTX治疗组和吡喹酮治疗组肝组织变性坏死及纤维化程度均较感染对照组和低剂量PTX治疗组轻.感染对照组、低剂量PTX治疗组、吡喹酮治疗组、高剂量PTX治疗组Bc1-2水平分别为(0.574±0.488%),(0.768±0.188%),(1,116±0.964%),(1.681±0.963%).高剂量PTX治疗组Bc1-2水平明显高于感染对照组和低剂量PTX治疗组(P<0.05),与吡喹酮组的表达水平相近(P>0.05).Bax水平分别为(0.372±0.291%),(0.351±0.249%),(0.465±0.356%),(0.79±0.642%).4组间Bax的表达水平无显著差异(P>0.05).结论:高剂量PTX可能通过促进Bc1-2表达,减少肝细胞的变性坏死,阻断血吸虫肝纤维化的发生.
AIM: To study the effects of pentoxifylline (PTX) on the expressions of hepatic Bcl-2 and Bax proteins in schistosomiasis mice with liver fibrosis METHODS: The model of schistosomiasis liver fibrosis in mice was established. The mice were divided into 4 groups: control group, high dose PTX group (360 g/kg Per day, ×8 wk), low dose PTX group (180 g/kg Per day, ×8 wk) and praziquantel treatment group (500 g/kg Per day,×2 d). Bcl-2 and Bax protein expressions were detected by immunohistochemisty. The pathological changes of liver were observed under microscope. RESULTS: The levels of Bcl-2 expression in control group, high dose PTX group, low dose PTX group and praziquantel treatment group were (0.574 ± 0.488%), (1.681 ± 0.963%), (0.768 ± 0.188%) and (1.116 ± 0.964%) respectively. The level of Bcl-2 expression in liver tissues of schistosomiasis mice with liver fibrosis in high dose group was significantly higher than that in low dose group (P<0.05) and controls (P<0.05). The level of Bax expression showed significant difference among the four groups. CONCLUSION: High doses of PTX could significantly increase expression of Bcl-2 in liver tissues of schistosomiasis mice with liver fibrosis by promoting Bcl-2 expression.
出处
《世界华人消化杂志》
CAS
2004年第10期2338-2340,共3页
World Chinese Journal of Digestology
基金
湖北省卫生厅科学研究资金资助项目
No.200019~~