血清HBV前S1蛋白检测的临床应用价值

Clinical application value of preS1 protein detection in HBV serum

目的 探讨血清 HBV前 S1蛋白检测的临床应用价值。方法 对 1 71例 HBs Ag(+)血清采用 ELISA方法检测其前 S1抗原及 HBV标志物 ,用荧光定量 PCR法检测其 HBV-DNA含量。结果 前 S1抗原在 HBc Ag(+)的标本血清中的检出率为 98.85 % (86/ 87) ,明显高于 HBe Ag(-)标本的 67.86% (5 7/ 84) ,两者之间存在极显著性差异 (χ2= 2 9.980 6,P<0 .0 1 ) ;前 S1抗原在 HBV-DNA(+)的标本血清中的检出率为 66.91 % (93 / 1 3 9) ,明显高于 HBV-DNA(一 )标本的 1 8.75 % (6/ 3 2 ) ,两者之间存在极显著性差异 (χ2 =2 4.745 9,P<0 .0 1 )。结论 前 S1蛋白在乙型肝炎的临床诊断及疗效观察等方面中起到重要的作用 ,对 HBV标志物及 HBV-DNA的检测有着重要的补充作用。

Objective To study the clinical application value of HBV preS1 protein detection.Methods The preS1 Ag and the HBV-M of 171 sera of patients with HBsAg-positive were detected by ELISA.And the HBV-DNA levels were by determined quantitative PCR.Results The preS1 Ag detectable rate of the sera with HBeAg-positive was 98.85%(86/87),distinctly higher than the that of 67.86%(57/84)of the sera with HBeAg-negative.The difference between them was marked(χ 2=29.9806,P <0.01);The preS1 Ag detectable rate in the sera with HBV-DNA-positive was 66.91%(93/139),distinctly higher than that of 18.75%(6/32) of the sera with HBV-DNA-negative.Statistic difference existed between them(χ 2=24.7459,P <0.01).Conclusions PreS1 protein detection plays a very important role in the diagnosis and curative effect observation of hepatitis B,ant it can be used as a proper supplement to conventionaltest of HBV-DNA and three pairs of Hepatitis B.