肿瘤热疗用F_2O_3纳米磁性粒子的生物相容性研究

The biocompatibility study of magnetic nanoparticles containing F_2O_3 used in tumor hyperthermia

目的 :研究将要用于肿瘤细胞内热疗的自制纳米级F2 O3磁性粒子的生物相容性。方法 :MTT法评价其体外细胞毒性 ,溶血试验评价其有无溶血作用 ,小鼠腹腔注射其无菌生理盐水混悬液以测定其LD50 ,微核试验评价其有无致畸、致突变作用等。结果 :该材料对L 92 9细胞毒性为 0～ 1级 ;无溶血作用 ;昆明小鼠腹腔注射该材料混悬液 1ml ,其LD50 为 5 .45g·kg- 1 ;微核实验结果表明无致畸、致突变作用。结论 :就目前所取得的实验结果来看 ,可认为该材料是一种具有良好生物相容性的材料 ,对于肿瘤热疗可能具有广阔的应用前景。

Objective To evaluate the in vitro and in vivo toxicity of selfprepared nanosized F 2O 3 ,which has the potential implication in tumor hyperthermia. Methods MTT assay was used to evaluate the in vitro cyitoxicity; hemolysis test was carried out to estimate blood toxicity; Fe 2O 3 suspended in sterile 0.9% NaCl were intraperitoneally injected into KM mouse to calculate the LD 50; micronucleus (MN) were reckoned to identify genotoxicity. Results The experiments showed that the toxicity of the material on mouse fibroblast (L929) cell lines was between 0～1 degree;it had no hemolysis activity; LD 50 arrived at 5.45g·kg -1 after intraperitoneal injection of 1ml suspension; micronucleus test showed it had no genotoxic effects either. Conclusions Our results show that the selfprepared nanosized F 2O 3 is a kind of high biocompatibility materials and perhaps is suitable for further application in tumor hyperthermia.