摘要
采用乙醚萃取法测定己酸菌液和黄水中的有机酸,在检测中发现,两台气相色谱仪所测出的测定值不相符,且差别较大。为了解决该问题,并保护色谱仪所配的INNOwax柱不受萃取液中乳酸的影响,使用现有的BP1柱专门用于测定黄水和发酵液中的酸。使用结果表明,色谱峰形略有前倾,但重现性好;BP1柱对极性物保留值小,若有较高沸点杂物滞留柱内,将柱温升到200℃以上即可;试样应多次进针,取平均值,多做重复测试,取3个以上数据平均值。
Aether extraction was applied to measure organic acids in caproate bacteria solution and in yellow water. However,the measured values by two gas chromatographs had evident difference. To settle this problem and to protect INNOwax chromatographic column not interfered by lactic acid in liquid extract, BP1 column was specially used to measure acids in yellow water and fermented solution, and the results indicated that chromatogram peak had slight pitch but good repeatability, BP1 column would result in small retention value of polar material, if there remained high boiling point materials in columns, temperature rise of column to above 200 ℃ could settle the problem. Repeated measurement should be done for samples to get average values. (Tran. by YUE Yang)
出处
《酿酒科技》
北大核心
2005年第1期72-73,77,共3页
Liquor-Making Science & Technology
关键词
分析检测
己酸发酵液
黄水
有机酸
气相色谱
analysis and detection
caproate fermented solution
yellow water
organic acid
gas chromatograph
