川芎嗪对大鼠支气管哮喘模型气道重塑的影响及机制

Effect of ligustrazine on airway remodeling in asthmatic rats

目的 观察川芎嗪对支气管哮喘 (简称哮喘 )大鼠模型气道重塑的抑制作用并探讨其作用机制。方法 32只SD大鼠按随机数字表法分成正常对照组 (A组 )、哮喘模型组 (B组 )、小剂量川芎嗪组 (C组 ,4 0mg/kg)和大剂量川芎嗪组 (D组 ,80mg/kg) ,以卵白蛋白 (OVA)致敏并长期吸入激发制备大鼠慢性哮喘模型。采用免疫组化半定量法测定气道壁胶原和转化生长因子 β1(TGF β1)含量 ,同时测定气道内、外径及平滑肌层、网状基底膜的厚度。结果 D组气道平滑肌层、网状基底膜的厚度为 (11 3± 1 3) μm、(11 3± 1 7) μm ,B组分别为 (19 7± 1 8) μm、(19 8± 1 6 ) μm ,两组比较差异有统计学意义 (P均 <0 0 1) ,但D组与A组 [(10 6± 1 2 ) μm、(9 8± 1 6 ) μm]、C组 [(11 6± 0 9) μm、(12 3± 1 8) μm]比较差异无统计学意义 (P均 >0 0 5 ) ;D组气道内外径比值为 0 77± 0 0 6 ,B组为0 6 3± 0 0 5 ,D组与B组比较差异有统计学意义 (P <0 0 1) ;D组气道壁Ⅲ型胶原及TGF β1含量吸光度 (A)值分别为 2 1± 5、2 6± 5 ,B组分别为 5 5± 7、6 9± 14 ,两组比较差异有统计学意义 (P <0 0 1) ,D组与C组 (32± 8、38± 10 )比较差异有统计学意义 (P <0 0 5 ) ,C组与B组比较差异也有统计学意义 (P <0

Objective To observe the effect and mechanism of ligustrazine on the airway remodeling Methods Thirty two SD rats were randomly divided into 4 groups: the normal group(A), the model group(B), the ligustrazine low dose group(C, 40 mg/kg) and the ligustrazine high dose group (D, 80 mg/kg), with 8 rats in each group The chronic asthmatic model was established by repeated inhalation of ovalbumin The changes of collagen and transforming growth factor β 1(TGF β 1) contents in the airway wall, the thickness of smooth muscle and basement membrane, inner and outer diameter were measured by the computerized image analysis system Results The thickness of smooth muscle and basement membrane were (11 3±1 3 , 11 3±1 7)μm in D group, (19 7±1 8, 19 8±1 6)μm in B group, the difference being significant ( P <0 01), as compared with A group [(10 6±1 2)μm, (9 8±1 6)μm] and C group [(11 6±0 9)μm, (12 3±1 8)μm], the difference being not significant (all P >0 05) The difference in the ratio of inner diameter to outer diameter was significant between D group (0 77±0 06) and B group (0 63±0 05), P <0 01 The contents of collagen type Ⅲ and TGF β 1 were (21±5, 26±5) in D group, (55±7,69±14) in B group, the difference being significant ( P <0 01) The differences were also significant when C group [32±8, 38±10] was compared with D group ( P <0 05) and B group ( P <0 01) The contents of collagen type Ⅰ showed no difference among the 4 groups (A group:34±13,B group:44±8, C group: 36±8, D group:39±8;all P >0 05) A close correlation between TGF β 1 and collagen type Ⅲ was demonstrated( r =0 844 2, P <0 01) Conclusion Ligustrazine might suppress airway remodeling by decreasing the expression of TGF β 1 and reducing deposition of collagen [