金鱼藻(Ceratophyllum demersum Kom.)对藻类的生化干预作用

BIOCHEMICAL INTERFERENCE OF AQUATIC MACROPHYTE CERATOPHYLLUM DEMBRSUM ON ALGAE

室内试验表明金鱼藻对普通小球藻(Chlorella vulgaris Beij.)和斜生栅藻(Scenedesmusobliquus(Turp.)Kutz.)的生长有明显的生化干预作用。本研究表明,金鱼藻和藻类共同培养,用培养过金鱼藻的培养液直接培养藻类,或用此培养液中提取的生物碱作藻类抑制实验,藻类的生长均受到抑制。等量的金鱼藻经煮沸后,对藻类的抑制作用高于原液。金鱼藻植株及其培养液中生物碱的含量与培养条件和时间有关。

Laboratory experiments have shown that C.demersum inhibited the growth of algae Chlorella vugaris and Scancdesmus obliguus. In culturing algae with C.demersum or the medium in which C.demerusm have grown already, the algae growth is inhibited.Alkaloids extracted from a medium in which C.demersum have been cult-ued were found to have an irhibitive effect on the growth of S.obliquus and the effect is larger than that of original medium. The boiled medium is still more inhibited than the unboiled. This may be owing to the presence of some antagonistic substances in the medium,or the extracting or boiling process have changed the nature of alkaloids.Rf value of alkaloids on the TLC plate (methanol:chlorofrom: ammonium hydroxide:water) are 0.89, 0.74 and 0.69, respectively, and on the plate (benzene:methanol) are 0.42, 0.31, and 0.15, respectively. Under UV-light, the spots showed a light yollow color.The contents of alkaloids in culture medium and plant body are related to the culture conditions.