慢性肾衰病人血清和红细胞抗氧化能力的ESR研究

ESR STUDIES ON THE ANTIOXIDATION PROPERTIES OF SERA AND ERYTHROCYTES FROM PATIENTS WITH ANEMIA OF CHRONIC RENAL FAIL

用电子自旋共振(ESR)自旋捕集技术研究了正常人和肾衰病人血清和红细胞对黄嘌呤 黄嘌呤氧化酶体系产生的氧自由基的作用.结果发现:(1)正常人血清和红细胞能够有效地清除超氧阴离子自由基(O_2^-),而肾衰病人血清和红细胞清除O_2^-的能力明显比正常人血清和红细胞低;(2)正常人血清能有效地把黄嘌呤 黄嘌呤氧化酶体系产生的O_2^-转化为·OH,病人血清在这方面与正常人血清有显著性差异.

Using electron spin resonance (ESR) spin trapping technique, we have studied the effects of sera and red blood cells from healthy people and patients with chronic renal failure on oxygen free radicals produced in xanthine xanthine oxidase system. Experimental results showed that: (1) sera and red blood cells from healthy people could both scavenge superoxide anion (O2) more effectively than sera and red blood cells from patients with chronic renal failure; (2) sera from healthy people could change (O2) to hydroxyl radical (OH) more easily than sera from the patients. The differences were significant by statistics two tail's t-test.