摘要
以桤叶唐棣的带芽茎段为外植体,对桤叶唐棣离体培养技术进行了初步研究,结果表明:初代培养基为MS+IAA0.1mg/L+6-BA0.5mg/L+KT0.5mg/L;增殖培养基为MS+IAA0.1mg/L+6-BA2.0mg/L,增殖芽数最大,达到5.4;GA3对茎芽长无促进作用;在1/4MS无激素培养基上,生根率达87.9%.
In order to study the best method for tissue culture and rapid propagation for Amelanchier alnifolia Nutt., stems with buds were chosen as explants. The results of in vitro culture showed that the shoot differentiation medium was MS+IAA 0.1 mg/L+6-BA 0.5 mg/L+KT 0.5 mg/L,the optimum medium for proliferation was MS+IAA 0.1 mg/L+6-BA 2.0 mg/L, GA_3 has no positive effect on the growth of shoot, the best medium for rooting was 1/4 MS without hormones, the rooting rate reached 87.9%.
出处
《西北植物学报》
CAS
CSCD
北大核心
2005年第2期400-404,共5页
Acta Botanica Boreali-Occidentalia Sinica
关键词
桤叶唐棣
组织培养
快速繁殖
Amelanchier alnifolia Nutt.
tissue culture
rapid propagation