药用植物青叶胆的组织培养

Tissue culture of medicinal plant Swertia mileensis

目的针对青叶胆野生资源受到严重破坏的情况,系统地探讨了通过组织培养为手段进行人工繁殖的方法。方法以幼茎和老叶为外植体,在MS培养基上添加不同的激素配比,改变培养方式。结果在所有实验方案中,对叶片来说,较适宜诱导愈伤组织的激素组合是Zt0.5mg/L+NAA0.1mg/L+IBA0.1mg/L或BA0.5mg/L+2,4-D0.1mg/L+IBA0.1mg/L,对幼茎来说,较适宜的诱导愈伤组织的激素组合则是BA0.02mg/L+Kt0.04mg/L+IBA0.05mg/L;对于芽的增殖,较适宜的激素组合是BA2.0mg/L+NAA0.5mg/L或BA2.0mg/L+IBA0.1mg/L;而根的诱导则在MS+Kt0.01mg/L+IBA0.5mg/L+NAA1.0mg/L培养基上进行。结论采用组织培养方式可进行青叶胆的快速繁殖,为确保这一珍稀药用植物资源的保护和可持续利用提供有效途径。

Objective In order to preserve the natural resources of Swertia mileensis which has been destroyed seriously, the method of artificial propagation by way of tissue culture has been systematically studied. Methods The immature stems and the mature leaves were the best material explants in rapid propagation in all of the experiments. These explants were cultured on MS culture media by adding different portions of hormones at various cultural conditions. Results For the leaves, the suitable phytohormone combination to induce callus are Zt 0.5 mg/L+NAA 0.1 mg/L+IBA 0.1 mg/L or BA 0.5 mg/L+2,4-D 0.1 mg/L+IBA 0.1 mg/L; for the stems, they are BA 0.02 mg/L+Kt 0.04 mg/L+IBA 0.05 mg/L; for the adventitious buds, they are BA 2.0 mg/L+NAA 0.5 mg/L or BA 2.0 mg/L+IBA 0.1 mg/L; and for the roots, they are MS+Kt 0.01 mg/L+IBA 0.5 mg/L+NAA 1.0 mg/L. Conclusion Tissue culture of S. mileensis can make its propagation rapid, its resources preserved, and its utilization last.