HIV感染长期不进展者与艾滋病患者HIV-1 gag特异CD_8^+T细胞应答

Human immunodeficiency virus-1 gag specific CD_8~ + T cell responses in long term nonprogressors and acquired immunodeficiency syndrome patients

目的 探讨欧美流行的人类免疫缺陷病毒 (HIV) 1B亚型株与我国HIV感染和艾滋病 (AIDS)病人 gag特异性CD+ 8T细胞应答交叉反应性。方法 研究对象为长期不进展者 (LTNP) 7例和艾滋病患者 9例 ,将覆盖HXB2HIV 1gag全长的 12 5个重叠肽段组成 11个肽段库作为抗原 ,用γ干扰素刺激原酶联免疫斑点试验方法检测LTNP和AIDS病人的特异性CD+ 8T细胞应答 ,观察两组病人间的差异及其与CD+ 4 T细胞和病毒载量的相关性。结果 LTNP组和AIDS组HIV 1gag特异性CD+ 8T细胞应答强度分别为 (12 12± 796 )斑点形成细胞数 (SFC) / 10 6外周血单个核细胞(PBMC)和 (182± 2 0 3)SFC/ 10 6PBMC ,识别肽段库的个数 (间接反应了细胞毒性T淋巴细胞应答的宽度 )分别为 3 0± 0 8和 0 8± 0 7,LTNP组显著高于AIDS组。CD+ 8T细胞应答的强度和宽度与CD+ 4 T细胞计数呈正相关 ,与病毒载量呈负相关。结论 欧美流行株与我国病毒株之间具有交叉反应性 ,HIV 1gag特异性CD+ 8T细胞应答在阻止疾病进展中可能发挥重要作用。

Objective To investigate the human immunodeficiency virus (HIV) 1 gag specific CD + 8 T cell responses in long term nonprogressors (LTNP) and acquired immunodeficiency syndrome (AIDS) patients in China Methods 7 LTNP and 9 AIDS patients were included in this study 11 peptide pools derived from 125 overlapping peptides spanning the full length of HXB2 gag were used as antigens HIV 1 specific CD + 8 T cell responses in these patients were examined by interferon γ ELISPOT assay, and investigated the relationship between HIV 1 gag specific CD + 8 T cell responses and CD + 4 T cell counts and the relationship between specific CD + 8 T cell responses and viral loads Results HXB2 could induce HIV 1 specific CD + 8 T cell responses in Chinese HIV/AIDS patients The strengths of HIV 1 gag specific CD + 8 T cell responses in LTNP and AIDS groups were (1212±796) SFC/10 6 PBMC and (182±203) SFC/10 6 PBMC, respectively The breadths of HIV 1 gag specific CD + 8 T cell responses of LTNP group and AIDS group were 3 0±0 8 and 0 8±0 7, respectively The values of these two parometers in LTNP group were significantly higher than that in AIDS group ( P <0 01) There was a positive relationship between the strength or the breadth of HIV 1 specific CD + 8 T cell responses and CD + 4 T cell counts, whereas there was a negative relationship between strength or breadth of CD + 8 T cell responses and viral loads Conclusions There was a cross reactivity between dominant HIV strain in Europe and America and those in China HIV 1 gag specific CD + 8 T cell responses may play a protection role during disease progression.