黄芩茎叶总黄酮对人宫颈癌Hela细胞株体外生长的影响

Effect of (SSTF) on human cervical carcinoma

目的探讨黄芩茎叶总黄酮(SSTF)治疗人宫颈癌的可行性。方法配制含人宫颈癌细胞的细胞悬 液(4×105个／ml),取96孔板,每孔接种细胞悬液100μl,阴性对照组设6个孔均不点药,阳性对照药为5-氟尿嘧 啶(5-Fu),取0.01、0.1、1、10、100μg／ml5个浓度,SSTF选取浓度与5-Fu相同,每个浓度点3个孔,37℃5％CO2 培养48-72小时后采用MTT法在酶标仪上以570nm波长测定吸光度(A),计算SSTF和5-Fu抑制Hela细胞 株体外生长的抑制率和半数抑制浓度(IC50)。结果 SSTF、5-Fu均可明显抑制肿瘤细胞的生长,其IC50分别为 20.5、10.5μg／ml。结论SSTF能抑制人宫颈癌Hela细胞体外生长,具有一定细胞毒性作用,可作为临床治疗宫 颈癌的药物进一步深入研究。

Objective To explore the feasibility of scutellarria baicalensis stem-leaf total flavonid (SSTF) on treating human cervical carcinoma. Methods The human cervical carcinoma cell (4 × 105/ml, 100μl) was added to 96 well plate. The negative group included 6 wells,the concentration of positive drug 5-Fu were 0. 01,0. 11,10,100μg/ml respectively as well as the SSTF. There were three wells at each concentration. The absorbency was assayed at 570nm wave on Enzyme-label instrument using MTT method,the IC50 and inhibiting rate of the growth of human cervical carcinoma cell in vitro were calculated. Results SSTF and 5-Fu could significantly inhibit growth of the tumor cell,IC50 was 20. 5μg/ml,10. 5μg/ml respectively. Conclusions SSTF and 5-Fu can significantly inhibit the growth of human cervical carcinoma cell in vitro and have certain cell toxicity. It is deserved to be further studied for treating human cervical carcinoma.