提高制备转基因小鼠效率的研究

Study on improving efficiency of producing transgenic mouse

目的 提高制备转基因动物的效率。方法 着重对注射DNA的制备 ,高质量受精卵的获得 ,显微注射及胚胎移植各步骤中的主要影响因素进行了优化。结果 移植后出生的小鼠经PCR及Sonthern Blot检测 ,从最初的 146只仔鼠中检测到 2只阳性鼠 ,提高到由 34只仔鼠中检测到 10只阳性鼠 ,阳性鼠概率由 1 4%基本稳定增加到 30 %。结论 通过技术改良后 ,转基因效率得到明显提高 。

Objective To improve the efficiency of producing transgenic mice.Methods The major steps were the preparation of DNA fragments for microinjection,the preparation of eggs with high quality,and the microinjection and implantation optimized.Results The born mice were examined by PCR and Southern Blot.At the beginning,we got 2 transgenic mice in 146 born mice.After improvement,we got 10 transgenic mice from 34 born mice.The ratio of transgenic founder mice was improved from 1.4% to 30%.Conclusion The technical platform for transgenic research has been establised due to the great improvement of the efficiency of producing transgenic mice.