小鼠卵母细胞体外成熟、体外受精的效果观察

In Vitro Maturation and Fertilization of Mouse Oocytes

目的 研究不同培养条件对小鼠卵母细胞体外成熟及体外受精率的影响。方法 小鼠卵母细胞分别在含有FSH、BSA和胰岛素的培养液中体外成熟,在Whitten 氏液中体外受精,比较体外成熟率、体外受精率。结果 1- 裸卵(DO) 的体外成熟率、体外受精率(81-4% ,31-0 % ) 均高于卵丘卵母细胞复合体(COC)(48-6 % ,27-1% ) 。2- 在培养液中添加FSH、胰岛素和BSA,卵母细胞的体外成熟率为77-9 % ,82-3% 、60-7% ;体外受精率为77-2 % 、72-6 % 、26-7% ;2 - 细胞率为49-2 % 、34-2 % 、10-0% 。胰岛素组的卵母细胞IVM 率最高,但IVF率、2 - 细胞率低于FSH 组。3- 添加BSA的两组的体外受精率只有26-7 % 、25-8 % ,显著低于其他组,其体外成熟率也较添加FSH 和胰岛素的组成。4- 排出第一极体(PbI) 的卵母细胞的体外受精率和2 - 细胞率(85-9 % ,22-4% ) 均高于GV期卵母细胞(71-1 % ,12-9 % ) 。结论 1- 卵丘卵母细胞(COC) 较裸卵(DO) 的体外成熟率、体外受精率都低,差异显著(P成熟< 0-01;P受精< 0-05) 。2-FSH 和胰岛素均能提高小鼠卵母细胞的体外成熟率、体外受精率。3-BSA可以降低小鼠卵母细胞体外受精率,差异极显著。4-GV 期卵母细胞的体外受精率显著低于体外培养的排出第一极体的卵母细胞(P2 - cell < 0-05,P受精<0-05)

To study mouse oocytes in vitro maturation and fertilization in different culture. Methods Compare the maturation and fertilization rate in vitro of mouse oocytes, which were culture in FSH, Insulin or BSA, and were fertilized in Whitter's medium. Results 1.IVM/IVF rate of Do (81.4%, 31.0%) were higher than those of COC (48.6%, 27.1%). 2.Mouse oocytes cultured with FSH, insulin and BSA, the IVM rate were 77.9%, 82.3% and 60.7%, the IVF rate were 77.2%, 72.6% and 26.7%, the 2-cell rate were 49.2%, 34.2% and 10.0% respectly. The group cultured with insulin (I-Group) have the highest rate of IVM, but after fertilization, fertilized oocytes and 2-cell embryos of I-Group are fewer than those cultured with FSH. 3.two groups cultured with BSA have lower rate in vitro fertilization rate (26.7%, 25.8%). 4.Fertilization and 2-cell rate in those oocytes with polar body 1 (85.9%, 22.4%) were higher than those of GV-stage oocytes (71.1%, 12.9%). Conclusions 1.In vitro maturation and fertilization rate of Do were significantly higher than those of COC (P m<0.01; P f<0.05). 2.The maturation and fertilization rate were improved when the mouse oocytes were cultured in FSH and Insulin mediums. 3.BSA affected the fertilization rate of mouse oocytes significantly. 4.The IVF rate and 2-cell rate of oocytes with PbI are higher than those of GV-stage oocytes (P f<0.05; P 2-cell <0.05).