脐血CD_3AK细胞杀伤白血病细胞的实验研究

Experimental Study of Cytotoxity Effect on Leukemia Cells by CD_3AK Cells from Cord Blood

目的 研究脐血CD_3AK细胞对白血病细胞的杀伤作用。方法 以抗CD_3单克隆抗体刺激脐血淋巴细胞产生CD_3AK细胞,以MTT法检测脐血CD_3AK细胞对K562细胞株的杀伤作用,并以正常入外周血单个核细胞作对照。结果 效靶比为10∶1,20∶16寸,脐血CD_3AK细胞对K562细胞和正常PBMC细胞的细胞毒活性分别为72.58±12.58％,18.68±10.84％,78.15±10.35％,20.28±11.46％。脐血CD_3AK细胞对K562细胞有显著的杀伤作用,而对正常PBMC无明显细胞毒作用(P<0.01)。结论 脐血CD_3AK细胞对K562细胞有高效杀伤作用,在肿瘤的过继性免疫治疗过程中有良好的应用前景。

Objective To study cytotoxity effect on leukemia cells by CD3AK cells from cord blood. Method We stimulated lymphocyte of cord blood with anti-CD3 monoclonal antibody toderive CD3AK cells, MTT method was used to detect cytotoxity effect on cell line K562 by CD3AK cell, mononuclear cells (MNC)from normal human blood were used as control. Result When ratio of effect cells to target cells is 10: 1 and 20 : 1, the activity of cytotoxity effect on K562, normal MNC by CD3AK is 73.58\12.58%, 18.68\10. 84%, 78.25 \10.35 %, 20.28\11.46% respectively. Conclusion CD3AK cells have obvious cytotoxity effect on K562 cells, but haven't obvious cytotoxity effect on normal MNC(P<0.01).