摘要
为了提取白桦三萜类化合物,建立了分光光度法测定白桦树皮中三萜类总量的分析方法。利用香草醛-高氯酸与三萜类化合物反应生成有色化合物的原理,以白桦脂醇为对照品,对白桦树皮中总白桦三萜类物质进行测定。结果表明:体系的最大吸收波长λ为551 nm,显色反应温度为70℃,反应时间为15 min;线性范围在1.0×10-2~3.0×10-2 μg/L;检测限为1.0×10-5 μg/L;精密度RSD为2.16%;回收率为96.16%~98.85%。
So as to abstract triterpenoids in betula,we set up spectrophotometric methods to determine the grand total of the triterpenoids in betula bark.Vanillirr-perchloric acid,glacial acetic acid react with betulin,served as detect sample to determine the grand total of the triterpenoids.The result of experiment showed that the maximum absorption of the system is 551 nm,the temperature of coloring is 70 ℃,reaction time is 15 min.The linear regression (equation) is A=9.198 5C+0.015 8,r=0.998 8.Linear range at 1.0×10^(-2)~3.0×10^(-2) μg/L. Detection limit is 1.0×10^(-5) μg/L.The precision RSD is 2.16%.The rate of recovery is (96.16%~)98.85%.
出处
《南京林业大学学报(自然科学版)》
CAS
CSCD
北大核心
2005年第1期110-112,共3页
Journal of Nanjing Forestry University:Natural Sciences Edition
基金
黑龙江省齐齐哈尔市科技局资助工业攻关项目(407036)