高氧液对急性光气中毒肺损伤保护作用的实验观察

Observation on the protective effect of hyperoxia solution on the acute lung injury caused by phosgene poisoning

目的观察家兔光气染毒后血气、生化指标及形态学的变化,研究高氧液对急性光气中毒性肺损伤的保护作用。方法18只新西兰大白兔随机分3组,每组6只,雌雄不拘。1组为空白对照组;另外2组动物吸入光气,其中一组(阳性对照组)静脉输入平衡盐,另一组(高氧液组)静脉输入高氧液,3组分别于实验的不同时间取血,测定动脉氧分压(PaO2)、血浆丙二醛(MDA)含量、超氧化物歧化酶(SOD)活力,8h后处死动物,取肺组织,测定肺组织中还原型谷胱甘肽(GSH)、氧化型谷胱甘肽(GSSG)含量,并做组织病理学检查。结果(1)高氧液组与阳性对照组动物光气中毒后血浆MDA含量都随实验时间的延长而升高,PaO2、SOD活力则呈下降趋势,肺组织GSH含量下降,GSSG含量升高。在1、3及8h时两组动物的血浆MDA含量、SOD活力及肺组织GSH、GSSG含量与空白对照组相比,差异有统计学意义(P<0.05或P<0.01)。(2)高氧液组在3、8h时(即在治疗后)PaO2分别为[(9.91±0.49)、(9.15±0.46)mmHg],均高于阳性对照组[(9.03±0.76)、(8.11±0.57)mmHg],差异有统计学意义(P<0.01);血浆MDA含量分别为[(3.66±0.35)、(5.31±0.15)μmol/L],均低于阳性对照组[(4.32±0.26)、(7.4±0.33)μmol/L],差异有统计学意义(P<0.01);红细胞SOD活力分别为[(237.37±29.96)、(208.10±18.80)

Objective To study the protective effect of hyperoxia solution on acute lung injury caused by phosgene poisoning by observing the changes of PaO_2 and malordialdehyde(MDA) contents、superoxide dismutase(SOD) activity in serum and Glutathione(GSH/GSSG) contents in lung tissues. Methods The rabbits were divided into normal control group,hyperoxia solution(H0) and balance salt(BS) groups.Group HO and Group BS inhaled phosgene and the former was given intravenously hyperoxia solution(which was replaced by balance salt solution in Group BS).The content of MDA and the activity of SOD in serum were observed at different time points,the amount of GSH and GSSG in lung tissue were also measured. Results (1)The serum MDA contents increased and PaO_2、SOD activity decreased significantly in Group HO and Group BS along with time increasing as compared with control group.The contents of GSH in lung tissue decreased in two groups compared with that in control group,however the contents of GSSG ascended instead.(2)At 3 and 8 h of the experiment,PaO_2 of Group HO[(9.91±~0.49), (9.15±0.46)mm Hg respectively]were significantly higher than those of Group BS[(9.03±0.76),^(8.11± 0.57)mm Hg respectively](P<0.01).The contents of MDA of Group HO(3.66±0.35)、^(5.31± ^0.15) μmol/L respectively] were lower than those of Group BS[(4.32±0.26)、(7.4±0.33)μmol/L respectively]^(P< 0.01).SOD acticity in Group HO[(237.37±29.96)、(208.10±18.80)NU/ml respectively] were higher than those of Group BS[(195.02±21.44)、(144.87±21.26)NU/ml respectively](P<0.05 or P<0.01).The content of GSSG lung tissue in Group HO(423.67±38.21)μmol/L were lower than those of Group BS(523.85±~43.14) mol/L(P<0.01).There were no significant differences in the content of GSH in lung tissues between Group HO and group BS. Conclution Hyperoxia solution can reduce acute lung injury of rabbits following phosgene poisoning.