甜菜夜蛾核型多角体病毒对拟澳洲赤眼蜂的影响:种群参数比较和PCR检测

Effect of Spodoptera exigua multicapsid nucleopolyhedrovirus on Trichogramma confusum: Comparison of population parameters and PCR detection

用生命表方法和PCR检测研究了甜菜夜蛾核型多角体病毒 (Spodopteraexiguamulticapsidnucleopolyhedrovirus,SeMNPV)对拟澳洲赤眼蜂TrichogrammaconfusumViggiani的影响。结果表明 ,在 2 5± 1℃下 ,取食含SeMNPV蜂蜜和纯蜂蜜 (对照 )的两组赤眼蜂雌蜂的寿命、繁殖力、羽化率、产雌率均无显著差异 ;内禀增长率 (rm)分别为 0 35 2 4和 0 3418,周限增长率 (λ)为 1 4 2和1 4 1,非常接近 ;净繁殖率 (R0 )分别为 12 4 34和 14 2 5 7,平均世代历期为 13 6和 14 5天 ,种群倍增时间分别为 1 96和 2 0 3天。对 5头取食含SeMNPV蜂蜜的雌蜂的子代的PCR检测结果也均为阴性。因此 ,结论认为SeMNPV对拟澳洲赤眼蜂成蜂并无明显影响 ,体内携带病毒的亲代蜂并不能将病毒传给子代。

The effect of (Spodoptera exigua multicapsid nucleopolyhedrovirus,SeMNPV) on Trichogramma confusum Viggiani was studied by the methods of life table and PCR. The results showed that there was no significant difference in population parameters of T. confusum females between the treatment with the mixture of SeMNPV-honey and that with honey alone at 25±1℃. For the treatment with the mixture of SeMNPV and the treatment with honey alone at 25±1℃, the intrinsic rate of increase (r_m) of T. confusum females was 0.3524 and 0.3418, respectively; the finite rate of increase (λ) 1.42 and 1.41; the net reproduction rate (R_0) 124.34 and 142.57; the mean generation time 13.6 and 14.5 days; the population doubling time 1.96 and 2.03 days. The results of PCR detection of the offsprings of 5 females of T. confusum which ingested the the mixture of SeMNPV-honey were all negative. It was therefore concluded that there was no distinct effect of SeMNPV on T. confusum adults, and T. confusum female adults infected with SeMNPV could not transmit SeMNPV to their offsprings.