盐胁迫下小麦甜菜碱和脯氨酸含量变化

Variation of Betaine and Proline Contents in Wheat Seedlings Under Salt Stress

运用高效液相色谱-质谱(HPLC-MS)联用技术 分析了耐盐性强、中、弱3个小麦品种SW12、宁春4 号和中国春苗期5个NaCl浓度胁迫下甜菜碱和脯氨酸 含量的变化。方差分析表明盐胁迫下3个小麦品种之间 甜菜碱的含量差异达到极显著水平(P<0.01),SW12的 含量最高,宁春4号次之,中国春最低,与小麦耐盐性 表现相一致;脯氨酸在叶片中的含量差异不显著,在 根中宁春4号和中国春的含量有显著差异(P<0.05)。结 果表明:小麦叶和根中甘氨酸甜菜碱含量与小麦盐胁 迫呈正相关,是小麦体内抵御盐胁迫的渗透调节物质 之一,可作为小麦耐盐性鉴定指标。

Glycine betaine (GB) and proline contents of leaf and root were simultaneously determined by HPLC-ESI-MS at seedling stage in the three wheat (Triticum aestivum L.) varieties (salt tolerance from high to low), SW12, Ningchun No.4 and Chinese Spring (C.S) under 5 different salt stress levels (Figs. 1,2). The GB contents among SW12, Ningchun No.4 and C.S were found outstanding difference by ANOVA (P< 0.01) and consistent with salt tolerance in wheat. Proline contents were not different among 3 wheat varieties in leaf, but difference was found in the root between the Ningchun No.4 and C.S (P<0.05). The result suggested GB, as one of the materials for osmotic adjustment in plant, had the closest relationship with salt tolerance and could be used as an index of salt tolerance in wheat.