摘要
目的通过检测重型乙型肝炎(乙肝)病人血清HBV特异性循环免疫复合物的含量,探讨HBV特异性循环免疫复合物与重型乙肝的关系。方法采用聚乙二醇穴PEG雪沉淀法制备HBV特异性免疫复合物阳性对照品,并对80例重型乙肝患者血清HBV特异性循环免疫复合物含量进行检测。结果80例重型乙肝患者血清解离前后HBsAg-IC阳性率最高穴56.25%雪。HBsAg-IC阳性与阴性者的年龄、HBsAg阳性率、ALT、TB水平等之间差别无显著性意义穴P>0.05雪,而HBVDNA、HBeAg阳性率之间差别有显著性意义(P<0.05)。结论PEG沉淀法检测HBV特异性循环免疫复合物简便、可靠,可用于临床检测;HBsAg-IC是重型乙肝患者循环免疫复合物的主要形式鸦HBsAg-IC阳性可能与病毒复制能力有关。
Objective To investigate the pathological significance of cir culating HBV specific immune complex in severe hepatitis B. Methods Serum HBV immune complex was detected by PEG (MV 6000) precipitation method in 80 patients with severe hepatitis B. Results Forty two of 80 (56.25%) cases showed HBsAg i mmune complex (HBsAg-IC) positive. There were statistically significant differen ces in HBV-DNA and HBeAg-positive rates between HBsAg-IC positive and HBsAg-IC n egative groups (P<0.05). Conclusion HBsAg-IC is the major circulating immune co mplex in severe hepatitis B and HBsAg-IC may be related to HBV replication.
出处
《浙江医学》
CAS
2005年第2期81-83,89,共4页
Zhejiang Medical Journal
基金
浙江省卫生厅重点课题(项目编号20020302)
