NGF对大鼠创伤性脑损伤后大脑皮质神经元的影响

The Effects of Nerve Growth Factor on Cerebrum Cortical Neurons in Vitro after Traumatic Brain Injury in Rats

目的 研究神经生长因子 (NGF)对大鼠创伤性脑损伤后大脑皮层神经元的影响 ,为NGF的临床应用提供实验依据。方法 用原代培养的胎龄 17dSD大鼠胎鼠大脑皮质细胞 ,机械划痕法建立体外创伤性脑损伤模型 ,通过细胞形态学、MTT法及琼脂糖凝胶电泳观察NGF的保护作用。结果 培养 10d的神经细胞行机械性损伤 ,神经细胞皱缩 ,突起消失 ,神经网络减少 ,胞浆内尼氏颗粒减少、溶解、消失 ,出现典型的DNA梯状断裂带 ;给予NGF后 ,NGF中、高剂量组倒置显微镜下及尼氏染色后观察细胞形态结构损伤减轻 ;MTT比色法数值增高 ;DNALadder无梯状断裂带出现。结论 NGF对创伤性脑损伤所致的大脑皮层神经细胞损伤具有保护作用。

Objective To study protective effect of nerve growth factor (NGF) on cerebrum cortical neurons in vitro after traumatic brain injury (TBI) in rats and provide experimental evidence for clinical application. Methods Primary cerebral cortical cultures from rat fetus after mechanical injury were used to observe the protective effects of NGF. The protective effects of NGF were evaluated by the method of MTT,histopathology and DNA ladder gel electrophoresis. Results Mechanical injuried primary cerebral cortical cultures elicited morphological changes including reduction in volume,disapeared dendrites and nissl body and contracted nerve net,decreased figure in MTT and DNA ladder electrophoresis through DNA gel electrophoresis. The NGF treat group with medium,high dose can reduce and reverse these changes. The activities of MTT in NGF group were statistically higher than that of TBI group. The morphological observation showed that the recovery in NGF groups was significantly better than that in TBI group. And no DNA ladder break fragmentation appeared in NGF group. Conclusions NGF can protect cerebrum neurons from mechanical injury.