大鼠肝纤维化过程中肝组织黏着斑激酶表达增加

Increase of the expression of FAK in rat liver tissue during hepatic fibrogenesis

本研究采用胆总管结扎 (BDL)方法建立大鼠肝纤维化模型 ,应用免疫组织化学、Westernblotting技术及逆转录聚合酶链式反应 (RT PCR)方法 ,研究了FAK及其mRNA在肝纤维化不同时期肝组织中的分布及含量的动态变化 ;用免疫组织化学方法测定α 平滑肌肌动蛋白 (α SMA)表达。结果显示 :正常肝组织有少量α SMA、FAK分布 ,随着肝纤维化发展 ,α SMA、FAK阳性细胞明显增多 ;正常大鼠肝组织中亦有FAK蛋白、FAKmRNA表达 ,造模 4周表达最多。FAK与α SMA呈显著正相关 (r=0.96 3,P <0.0 5 )。提示肝纤维化形成过程中FAK及其mRNA表达明显增加 。

In order to observe changes of the expression of FAK and FAK mRNA in fibrotic rat liver, which was induced by bile duct ligation (BDL), Western blotting and reverse transcription-polymerase chain reaction (RT-PCR) was used to determine the expression of FAK on both protein and mRNA,while the distributions of FAK in the livers were assessed by Immunohistochemistry. The liver histopathological changes were evaluated by hematoxylin and eosin staining,and by Masson's trichrome method. Besides, numbers of activated HSCs were quantified after alpha smooth muscle actin(α-SMA) staining. With the development of hepatic fibrosis,the positive cells of α-SMA increased obviously. They mainly resided in the cells of portal ducts,fiber septa,perisinuses and around the proliferated bile ducts. The positive areas of the rat liver in model groups at week 1 to 4(12.88%±2.63%,22.65%±2.16%,27.45%±1.86%,35.25%±2.34%)were larger than that in the control group(5.88%±1.46%),P<0.01. The positive cells of FAK increased a lot,they were mainly distributed in portal ducts,fiber septa and around the bile ducts,vascular endothelial cells and perisinusoidal cells. FAK correlated with α-SMA positively,r value was 0.963,P<0.05. FAK protein and FAK mRNA expression was up-regulated in model groups. The initial increasing began at 2 d and reached its peak 4 weeks after operation. Therefore, for increase of FAK and FAK mRNA expressions might be linked to hepatic fibrogenesis.