尼尔雌醇和左炔诺孕酮对人成骨肉瘤MG-63细胞株OPG/OPGL的作用

Effect of nylestriol and levonorgestrel on the expression of OPG/OPGL in human osteosarcoma MG-63 cell lines

目的 :观察不同浓度的尼尔雌醇 (NYL)和左炔诺孕酮 (LNG)对MG 6 3细胞株OPG/OPGL表达的作用 ,探讨旁分泌效应对其表达的影响。方法 :用半定量RT PCR检测不同浓度的两种药物分别干预的MG 6 3细胞OPG/OPGLmRNA表达。结果 :两种药物均能促进MG 6 3细胞株OPG表达增多 ,最佳作用浓度均为 10 6mol/L。两者均能显著抑制OPGLmRNA表达。更换培养液可使NYL (10 10 mol/L)干预组表达OPG减少。结论 :NYL和LNG均能促进MG 6 3细胞株OPG表达 ,并抑制OPGL的表达。NYL对OPG表达调控过程中可能涉及MG 6

Objective To determine the effect of nylestriol and levonorgestrel on mRNA expression of OPG/OPGL in MG-63 cell lines,and to explore whether the paracrine effect is involved in the regulation course. Methods Semi-quantitive RT-PCR was conducted to analyze mRNA expression of OPG/OPGL in MG-63 cell lines treated with nylestriol and levonorgestrel.Results Both medicines could stimulate MG-63 cell lines to express OPG, and the best action levels of the two medicines were 10 -6 mol/L.Both medicines could decrease mRNA expression levels of OPGL dramatically. In NYL 10 -10 mol/L treatment group, medium renewal has negative effect on mRNA expression of OPG.Conclusion Both the two medicines can promote mRNA expression of OPG in MG-63 cell lines, while decrease mRNA expression of OPGL.The paracrine effect of MG-63 cell lines may be involved in the regulation of mRNA expression of OPG by NYL.