加减温胆汤对抑郁模型大鼠行为学和血浆生长抑素含量的影响

Effect of Jiajian Wendan Tang on behavior and plasma content of somatostatin in rats with depression

目的:通过以调理脾胃法组成的中药复方加减温胆汤对抑郁大鼠行为学和血浆生长抑素含量的影响,探讨其发病机制。方法:实验于2004-03/04在广西中医学院中心实验室完成。以慢性不可预见性应激诱导SD大鼠抑郁模型,观察中药复方加减温胆汤的抗抑郁效应,采用旷场实验测定其水平活动格数和垂直活动次数,以放免法测定血浆生长抑素含量,并与正常组、模型组、西药组相对照。结果:中药高、低剂量组犤(182.70±55.60),(147.70±58.20)ng/L犦和西药组(169.90±48.89)ng/L大鼠血浆生长抑素含量均高于模型组(84.10±23.67)ng/L(q=3.33～5.16,P<0.05～0.01),中药高剂量组效果更为显著(q=5.16,P<0.01)。中药高、低剂量组和西药组旷场活动明显增强,与模型组相比,差异有显著性意义(P<0.05,P<0.01)。结论:加减温胆汤具有抗抑郁作用,其作用机制可能通过调节脑和血浆内生长抑素水平,达到抗抑郁目的。

AIM:To investigate the effect of Jiajian Wendan Tang(modified decoction for clearing away gallbladder-heat),which is a Chinese compound prescription with the function of adjusting spleen and stomach,on the behavior and plasma content of somatostatin in depressive rats. METHODS:The experiment was accomplished in the central laboratory of Guangxi College of Traditional Chinese Medicine from March to April 2004.SD rat models of chronic stress-induced depression were used to observe the antidepressant action of Jiajian Wendan Tang.Open-field test was applied to measure the score of horizontal motion and vertical motion,and plasma content of somatostatin was determined with radioimmunoassay,and then the results were compared with those in the normal group,model group and western medicine group. RESULTS:The plasma contents of somatostatin in the high and low dose Jiajian Wendan Tang groups [(182.70±55.60),(147.70±58.20) ng/L] and western medicine group[(169.90±48.89) ng/L] were all higher than that in the model group[(84.10±23.67) ng/L] (q=3.33 to 5.16,P< 0.05 to 0.01),and the curative effect was more significant in the high dose Jiajian Wendan Tang group(q=5.16,P< 0.01).The results of open-field test in the high and low dose Jiajian Wendan Tang groups were significantly enhanced as compared with those in the model group(P< 0.05,P< 0.01). CONCLUSION:Jiajian Wendan Tang has the effect of antidepression, and its mechanism may be related to the adjustment of content of somatostatin in plasma and brain.