香樟离体培养体系的构建初探

Initial Culture of Cinnamomum caphora (L.) Presl in vitro

以香樟茎段为试验材料,采用正交试验设计方案,研究了大量元素、BA和NAA三因素对初步建立香樟茎段离体培养体系的影响。接种30d后,培养基中的香樟茎段均有程度不同的器官发生现象。统计分析结果表明:大量元素、BA和NAA均对香樟茎段隐芽的发育以及愈伤组织的产生有显著影响。全量的MS大量元素适于香樟茎段的隐芽发育。BA/NAA浓度比为40时适于芽的萌出,为5时适于愈伤组织产生。在笔者试验中,9号即MS+BA2.0(mg/L)+NAA0.05(mg/L)培养基为香樟茎段隐芽萌动与伸长的最适培养基,7号即1/2MS+BA0.5(mg/L)+NAA0.1(mg/L)培养基为香樟茎段产生愈伤组织的最适培养基。在进一步工作中还可加大BA浓度促进芽的萌发,增加NAA浓度或降低BA浓度促进愈伤组织产生。

In order to get the optimal medium to culture Cinnamomum caphora (L.) Prest. in it, semi-ligneous stem sections of a dominant tree in a mature forest were used as explants and orthogonal layout L9(34) with the compound of three elements (macroelements, BA and NAA) with three levels was adopted in this study. Four weeks later, either axillary sprouts or callus or both were produced by the stem sections cultured in all media. The results showed that the tested three factors have significantly affected the growth of sprouts and callus. Macroelements in MS medium and the highest concentration of BA were the optimal for the growth of sprouts, whereas the optimal conditions to develop callus were the highest concentration of NAA and the lowest concentration of BA. In addition, the sprouts flourished when the ratio of BA/NAA was equal to 40. However, the optimal value of BA/NAA to develop callus was 5 which was the lowest one among the ratios. Although the optimal medium to develop axillary sprouts was MS+BA2.0(mg./L)+NAA0.05(mg./L) and the optimal medium to produce callus was 1/2MS+BA0.5(mg./L)+NAA0.1(mg./L) in this study, the concentration of BA could be increased to develop more sprouts and we could get more callus by increasing concentration of NAA either lower concentration of BA for further study.