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核黄素缺乏大鼠红细胞膜流动性与脂质过氧化关系的研究 被引量:16

THE RELATIONSHIP OF ERYTHROCYTE MEMBRANE FLUIDITY WITH LIPID PEROXIDATION IN RIBOFLAVIN DEFICIENT RAT
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摘要 对两组大鼠分别喂饲核黄素缺乏(RD)膳和核黄素添加(RS,22mg/kg饲料)膳。7周后,测定了大鼠红细胞(RBC)膜荧光偏振度(P)和平均微粘度(?),RBC膜丙二醛(MDA)及RBC超氧化物歧化酶(SOD)。结果表明:RD组大鼠RBC膜P值和(?)值(分别为0.2976±0.0198和3.9483±0.3680)均高于RS组(0.2760±0.0207和2.8753±0.4634),差别分别呈显著和极显著统计学意义(P<0.05,P<0.01)。而RD组的RBC膜MDA(0.6868±0.1732nmol/mg蛋白质)和RBC SOD(7.7452±0.0610nU/mg蛋白质)则分别高于和低于RS组(0.5548±0.0980nmol/mg蛋白质和8.2685±0.3010nU/mg蛋白质),差别均有显著统计学意义(P<0.05)。研究表明:核黄素缺乏可引起大鼠RBC膜流动性下降,并与RBC膜脂质过氧化加重、清除自由基能力下降有关。 The effect of riboflavin deficiency on the fluidity of erythrocyte (RBC) membrane, the level of membrane MDA, the activity of RBC SOD and lipid peroxidation were studied. Growing male rats were fed with an experimental (riboflavin-deficient, RD) or a control (riboflavin-supplemented, RS, 22mg/ kg) diet for 7 weeks.The RBC membrane of RD rats contained higher level of MDA (0.6868±0.1732 nmol/mg protein) compared with the control (0.5548±0.0980 nmol/mg protein), while the activity of RBC SOD (7.745210.6101 nU/mg protein) of RD rats were significantly decreared compared with the control (8.2685±0.3010 nU/mg protein), which indicated the lipid peroxidation was elevated.Membrane fluidity was studied with fluorescence polarization (P) and mean microviscosity (η) using DPH as probe. The value of P andη of RD rats (0.2976 ± 0.0198, 3.9483± 0.3680, respectively) were significantly higher than the RS rats (0.2760 ± 0.0207, 2.875310.4634, respectively), which showed the membrane fluidity in RD rats was decreased according with the increase in lipid peroxidation. This study demonstrated that the RBC membrane fluidity was dicrcased in riboflovin deficient rats. It was relative to the increasing lipid peroxidation and decreasing RBC SOD activity.
出处 《营养学报》 CAS CSCD 北大核心 1993年第4期407-410,共4页 Acta Nutrimenta Sinica
关键词 核黄素缺乏 膜流动性 过氧化脂质 riboflavin deficiency membrane fluidity lipid peroxidation
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  • 1季健平,南京铁道医学院学报,1991年,10卷,27页
  • 2团体著者,中华医学杂志,1989年,69卷,663页
  • 3曹锡清,生物化学与生物物理进展,1986年,13卷,17页
  • 4聂松青,北京医科大学学报,1983年,15卷,249页
  • 5李益新,生物化学与生物物理进展,1983年,10卷,38页

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