脐血清GM-CSF、IL-3和IL-6水平检测及意义

Level and Significance of GM-CSF,IL-3 and IL-6 in Human Cord Blood Serum

目的：探讨脐血的造血支持作用及其作用机理，为脐血的临床应用提供理论依据。方法：采用固相ＥＬＩＳＡ法检测３４例脐血清和２０例成人血清的ＧＭ－ＣＳＦ、ＩＬ－３和ＩＬ－６含量。结果：脐血清ＧＭ－ＣＳＦ、ＩＬ－３和ＩＬ－６含量分别为１９．１±１４．８、１９．２±１２．７和５７．５±２９．１ｐｇ／ｍｌ，成人血清ＧＭ－ＣＳＦ和ＩＬ－６未测出，ＩＬ－３含量为２．２±２．９ｐｇ／ｍｌ，差异均有极显著意义（Ｐ均＜０．００１）。结论：脐血含有丰富的造血生长因子，可用于再生障碍性贫血和化疗等原因引起的粒细胞减少的治疗。

Objective:To explore the mechanism of human cord blood(CB)on stimulating hematopoiesis.Methods:Solid phase sandwich enzyme linked-immunosorbent assay was carried out to investigate the levels of granulocytc-macrophagc colony-stimulating factor(GM-CSF),interlcukin(IL-3)and IL-6 for 34 CB sera and 20 adult sera.Results:There were significantly higher concentrations of GM-CSF,IL-3 and IL-6 in CB (19.1±14.8;19.2±12.7;57.5±29.1 pg/ml)compared with that in adult sera(GM-CSF and IL-6 were undetectable;2.2±2.9 pg/ml for IL-3)(all P<0.001). Conclusion:There are higher up-regulating hematopoietic growth factors acting on early hematopoietic stem/progenitor cells in CB.CB may be useful for treatment of aplastic anemia and chemotherapy-induced granulopenia.