羊牛布鲁氏菌对C_(57)BL小鼠的自然杀伤细胞活性的影响

Effect of two strains of Brucellae on the NK cell activity in C_(57)BL mice.

通过应用~3H—TdR 释放法检测了羊、牛两种布鲁氏菌感染后 C_(57)BL 小鼠的 NK 细胞活性。发现用5×10~4菌量的羊种布鲁氏菌16M 皮下途径感染小鼠后第1天和第3天时 NK 细胞活性明显降低(P<0.01),感染后5～30天时的 NK 细胞活性与对照组相比无显著性差异(P>0.05)。16M 引起小鼠的 NK 细胞活性的降低与感染菌量的大小有关。16M 通过皮下和腹腔两种途径感染小鼠均能引起其 NK 细胞活性的降低(P<0.01)。弱毒牛种布鲁氏菌104M 5×10~4菌量接种后第3天时,小鼠的 NK 细胞活性与对照组相比无显著性差异(P>0.05)。

The NK cell activity in C_(67)BL inbred mice infected with Brucellae was studied by using ~3H-TdR release as-say.We found that there was an outstanding descent(P<0.01)of NK activities 1 day and 3 days after infectionwith 5×10~4 colony forming unit(CFU)/0.5ml of virulent Br.melitensis 16 by s.c.route,or 3 days after chal-lenged through i.p.route with 5×10~7 CFU/0.5ml of 16M.But no significant change of NK cell activities was foundwhen challenge with 5×10~3 CFU/0.5ml of 16M 1 day after infection,or challenge with 5×10~4 CFU/0.5ml of at-tenuated Br.abortus strain 104M 3 days after infection.The results provide preliminary data for basic studies of the pathogenesis of Brucellosis.