摘要
从猪胰制取测定甘油三酯(TG)的脂肪酶,其活性大于2.7×10~4u/mg;比活为5.5×10~4u/mg 蛋白;酶作用的最佳 pH、最适反应时间及最适温度分别为7.5~10.0、15min 及37~50℃。聚丙烯酰胺梯度凝胶电泳测得分子量为34700及20900;以橄榄油为底物的米氏常数为0.77%。
Lipase with high specificity for the determination of triglyceridewas prepared from porcine pancreas.The specific activity of the enzyme was5.5×10~4u/mg protein and the optimum pH,time and temperature for the en-zymic reaction were 7.5~10.0,15 min and 37~50℃,respectively.The molecularweight of the product was 34700 and 20900 and the Michaelis constant,deter-mined by use of olive oil as substrate,was 0.77%.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
1993年第1期1-3,共3页
Chinese Journal of Pharmaceuticals
关键词
脂肪酶
胰
甘油三酯
测定
猪
制备
lipase
pancreas
triglyceride
determination