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肿瘤浸润性淋巴细胞的分离、培养及其表型分析 被引量:7

STUDIES ON THE SEPARATION, CULTURE AND SURFACE MARKER OF TUMOR-INFILTRATING LYMPHOCYTES (TIL)
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摘要 本文运用不连续密度离心的方法从鼠B16黑色素瘤体中分离到肿瘤浸润性淋巴细胞(TIL),并对TIL的体外培养的条件及细胞性质进行了研究。TIL能在含IL-2的培养基中长期培养,且可不断扩增至一定数量,经液氮冻存的TIL仍可复苏、存活.在含IL-2的培养基中,TIL的扩增能力强于LAK细胞.生长TIL为T淋巴细胞.TIL的细胞表型随培养时间而有变化,即培养初期至30天内以Lyt-2^+细胞(Tc/Ts)占多数,以后则以L3T4^+细胞(T_H)为主.本文建立的TIL的分离、培养技术可靠、易行,为开展TIL的肿瘤过继免疫疗法的研究提供了条件. The separation of tumor-infiltrating lymphocytes (TIL) from tumor mass of B16 melanoma was achieved by discontinuous density gradients centrif ugation and the in vitro culture of TIL were investigated. Long-term culture and expansion of TIL were accomplished in complete medium containing 500u/ml IL-2. TIL would grow as usual when it was thawn after cryopreservation. TIL exhibited a far better expansion capability than LAK cells. Our data demonstrated changes in the phenoty-pe of TIL during the culture period. Higher Lyt-2+ cell and lower L3T4+ cell counts were seen in the early period and L3T4+ cells incressed to be the majority in the later stage of culture. The method described in this paper was effective and practical. It provides a basis for studying the tumor adoptive immunotherapy with TIL.
出处 《上海免疫学杂志》 CSCD 北大核心 1989年第5期262-266,共5页 Shanghai Journal of Immunology
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